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| Status |
Public on Jun 20, 2024 |
| Title |
Single Nucleus DNA Sequencing of Flow Sorted Archived Frozen and Formalin Fixed Paraffin Embedded Solid Tumors |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Archived tissues are a vast resource of annotated clinical samples. To expand their use for translational studies, we optimized flow sorting and sequencing of single nuclei from archived frozen and FFPE tumor tissues. Our methods, which include isolation and preparation of intact nuclei suitable for library preparations, quality control (QC) metrics for each step, and a single cell sequencing bioinformatic processing and analysis pipeline, were validated with flow sorted nuclei from matching frozen and FFPE ovarian cancer surgical samples and a sequencing panel of 553 amplicons targeting single nucleotide and copy number variants in genes of interest.
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| Overall design |
frozen and FFPE matching paired ovarian cancer tissue
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| Contributor(s) |
Chapman J, Barrett M, Zhou Y, Chen X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA1124378 |
| |
| Submission date |
Jun 20, 2024 |
| Last update date |
Jun 21, 2024 |
| Contact name |
Xianfeng Chen |
| E-mail(s) |
chen.xianfeng@mayo.edu
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| Organization name |
Mayo Clinic
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| Street address |
13400 E Shea Blvd
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| City |
Scottsdale |
| State/province |
AZ |
| ZIP/Postal code |
85259 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (16)
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