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| Status |
Public on Jun 21, 2024 |
| Title |
Genetic diversity accelerates canine distemper virus adaptation to ferrets |
| Organisms |
Mustela putorius furo; Chlorocebus sabaeus; canine distemper virus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA viruses adapt rapidly to new host environments by generating highly diverse genome sets, so-called “quasispecies”. Minor genetic variants promote their rapid adaptation allowing for emergence of drug-resistance or immune-escape mutants. Understanding these adaptation processes is highly relevant to assess the risk of cross-species transmission, and safety and efficacy of vaccines and antivirals. We hypothesized that genetic memory within a viral genome population facilitates rapid adaptation.
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| Overall design |
We investigated the adaptation of the Morbillivirus canine distemper virus (CDV) to ferrets and compared an attenuated, Vero cell-adapted virus isolate (5804) with its recombinant derivative (r5804) over consecutive in vivo passages in ferrets. We performed RNA-seq analyses of infected organ homogenates and determined the mutant spectra within the CDV genome populations replicating in these tissues at each passage.
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| Contributor(s) |
Siering O, Langbein M, Herrmann M, Wittwer K, Sawatsky B, von Messling V, Pfaller CK |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA1124512 |
| |
| Submission date |
Jun 21, 2024 |
| Last update date |
Jun 22, 2024 |
| Contact name |
Christian Karl Pfaller |
| E-mail(s) |
pfaller.christian@mayo.edu
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| Organization name |
Mayo Clinic
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| Department |
Molecular Medicine
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| Street address |
200 First Street SW
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| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
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| Platforms (2) |
| GPL34629 |
Illumina HiSeq 4000 (canine distemper virus; Chlorocebus sabaeus) |
| GPL34630 |
Illumina HiSeq 4000 (canine distemper virus; Mustela putorius furo) |
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| Samples (9)
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| GSM8343105 |
CDV-infected VerodogSLAMtag cells (passage 0) |
| GSM8343106 |
CDV-infected ferret organ homogenate (passage 1) |
| GSM8343107 |
CDV-infected ferret organ homogenate (passage 2) |
| GSM8343108 |
CDV-infected ferret organ homogenate (passage 3) |
| GSM8343109 |
recombinant CDV-infected VerodogSLAMtag cells (passage 0) |
| GSM8343110 |
recombinant CDV-infected ferret organ homogenate (passage 1) |
| GSM8343111 |
recombinant CDV-infected ferret organ homogenate (passage 2) |
| GSM8343112 |
recombinant CDV-infected ferret organ homogenate (passage 3) |
| GSM8343113 |
recombinant CDV-infected ferret organ homogenate (passage 6) |
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