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| Status |
Public on Jun 25, 2024 |
| Title |
Pathogen-induced tissue-resident memory TH17 (TRM17) cells amplify autoimmune kidney disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Although it is well established that microbial infections predispose to autoimmune diseases, the underlying mechanisms remain poorly understood. After infection, tissue-resident memory T (TRM) cells persist in peripheral organs and provide immune protection against reinfection. However, whether TRMcells participate in responses unrelated to the primary infection, such as autoimmune inflammation, is unknown. By using high-dimensional single-cell analysis, we identified CD4+TRMcells with a TH17 signature (termed TRM17 cells) in kidneys of patients with ANCA-associated glomerulonephritis. Experimental models demonstrated that renal TRM17 cells were induced by pathogens infecting the kidney, such asStaphylococcus aureus,Candida albicans, and uropathogenicEscherichia coli, and persisted after the clearance of infections. Upon induction of experimental glomerulonephritis, these kidney TRM17 cells rapidly responded to local proinflammatory cytokines by producing IL-17A and thereby exacerbate renal pathology. Thus, our data show that pathogen-induced TRM17 cells have a previously unrecognized function in aggravating autoimmune disease.
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| Overall design |
scRNA-seq of human samples from the kidney and peripheral blood, specifically from individuals with ANCA-associated glomerulonephritis, was performed from FACS-sorted CD3 positive T cells using Single Cell 3' v2 (10x Genomics) according to manufacturer's instructions. Libraries were sequenced aiming at 50,000 reads per cell on an Illumina HiSeq4000.
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| Contributor(s) |
Zhao Y, Krebs CF, Panzer U, Bonn S |
| Citation(s) |
32769171 |
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| Submission date |
Jun 24, 2024 |
| Last update date |
Jun 27, 2024 |
| Contact name |
Christian Krebs |
| E-mail(s) |
c.krebs@uke.de
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| Organization name |
UKE
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| Street address |
Martinistraße 52
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| City |
Hamburg |
| State/province |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (18)
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| GSM8346285 |
Patient 04, Kidney |
| GSM8346286 |
Patient 05, Kidney |
| GSM8346287 |
Patient 06, Kidney |
| GSM8346288 |
Control 01, Kidney |
| GSM8346289 |
Control 02, Kidney |
| GSM8346290 |
Control 03, Kidney |
| GSM8346291 |
Patient 01, Blood |
| GSM8346292 |
Patient 02, Blood |
| GSM8346293 |
Patient 03, Blood |
| GSM8346294 |
Patient 04, Blood |
| GSM8346295 |
Patient 05, Blood |
| GSM8346296 |
Patient 06, Blood |
| GSM8346297 |
Control 01, Blood |
| GSM8346298 |
Control 02, Blood |
| GSM8346299 |
Control 03, Blood |
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| Relations |
| BioProject |
PRJNA1127463 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE270533_RAW.tar |
3.4 Gb |
(http)(custom) |
TAR (of H5, RDS) |
SRA Run Selector |
| Raw data are available in SRA |
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