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| Status |
Public on Aug 15, 2024 |
| Title |
Characterization of gene profile between ALDH high LNCaP cells and ALDH low LNCaP cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Acquisition of prostate cancer stem cells (PCSCs) manifested during androgen ablation therapy (ABT) contributes to castration-resistant prostate cancer (CRPC). However, little is known about the specific metabolites critically orchestrating this process. Here, we show t+B13hat IMPA1-derived inositol enriched in PCSCs is a key metabolite crucially maintaining PCSCs for CRPC progression and ABT resistance.
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| Overall design |
For fluorescence-activated cell sorting, cells were labeled with ALDEFLUOR Kit and sorted using MoFlo Astrios Cell Sorter (BECKMAN COULTER) in two ways including highest 10 % ALDH positive cells (ALDHhigh) and the lowest 10% ALDH negative cells (ALDHlow/-), following by cell culture and immunoblotting.
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| Contributor(s) |
Hsu C |
| Citation missing |
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| |
| Submission date |
Jun 24, 2024 |
| Last update date |
Aug 15, 2024 |
| Contact name |
Che-Chia Hsu |
| E-mail(s) |
che-chia.hsu@duke.edu
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| Phone |
9196682177
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| Organization name |
Duke University
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| Department |
Pathology
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| Street address |
210 Research Drive, GSRB2, Rm#4014
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL34281 |
Illumina NovaSeq X (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1127501 |
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