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| Status |
Public on Sep 29, 2024 |
| Title |
Holliday junction resolvase RuvC targets biofilm eDNA to facilitate pathogen spread in plants |
| Organism |
Ralstonia solanacearum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Biofilm lifestyle is critical for bacterial pathogens to colonize and protect themselves from host immunity and antimicrobial chemicals in plants and animals. The formation and regulation mechanism of phytobacterial biofilm are still obscure. Here, we found that Ralstonia solanacearum Resistance to ultraviolet C (RuvC) is highly abundant in biofilm and positively regulates pathogenicity by governing systemic movement in tomato xylem. RuvC protein accumulates at the later stage of biofilm and specifically targets the Holliday junction (HJ) like structures to disrupt biofilm extracellular DNA (eDNA) lattice, thus facilitating biofilm dispersal. Recombinant RuvC protein can resolve extracellular HJ prevent bacterial biofilm formation. Heterologous expression of R. solanacearum or Xanthomonas oryzae pv. oryzae RuvC with plant secretion signal in tomato or rice confers resistance to bacterial wilt or bacterial blight disease, respectively. Plant chloroplast localized HJ resolvase monokaryotic chloroplast 1 (MOC1) which is structural similar to bacterial RuvC shows a strong inhibit effect on bacterial biofilm formation. Re-localization of SlMOC1 to apoplast in tomato roots leads to increase resistance to bacterial wilt. Our novel finding reveals a critical pathogenesis mechanism of R. solanacearum and provides an efficient biotechnology strategy to improve plant resistance to bacteria vascular disease.
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| Overall design |
The sample names were GMI1000 and ΔRuvC, and the source of the sample species was R.solanacearum. The wild-type GMI1000 or ΔRuvC mutant was cultured to OD600=1 at 28℃. Different single colonies were as 1 biological replicate, and total of 3 biological replicates were performed.
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| Contributor(s) |
Du X, Fan C, Li P, Tian J, Lin Y, Xie J, Cheng J, Fu Y, Jiang D, Yuan M, Yu X, Tsuda K, Li B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 25, 2024 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Xinya Du |
| E-mail(s) |
duxinya9869@163.com, xinyadu@webmail.hzau.edu.cn
|
| Phone |
18602761757
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| Organization name |
Huazhong Agricultural University
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| Street address |
Huazhong Agricultural University, No. 1, Shishishan Street, Hongshan District
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
| GPL34644 |
Illumina NovaSeq X Plus (Ralstonia solanacearum) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1128091 |
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