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| Status |
Public on Sep 10, 2024 |
| Title |
Increased Autoreactivity and Maturity in EBI2+ Antibody-Secreting Cells from Nasal Polyps |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Rationale: Elevated numbers of antibody secreting cells (ASCs) and anti-dsDNA antibodies are found in nasal polyp (NP) tissue. Tissue anti-dsDNA IgG prospectively predicts recurrent NP, but the characteristics of the source ASCs are unknown. Objectives: To investigate whether NP B cells expressing the extrafollicular marker EBI2 have increased propensity for autoantibody production and to evaluate the molecular characteristics of NP ASCs. Findings: NPs (n=8) showed increased frequencies of anti-dsDNA IgG and total IgG ASCs (34- and 9-fold, respectively, both p<0.0001) compared to tonsils (n=10), with more pronounced differences among EBI2+ cells. In NPs, EBI2+ cells were frequently double-negative (IgD-CD27-) and ASCs (36.3% and 19.5%, respectively). ScRNA-seq analysis of tonsils and NPs (both n=5) revealed substantial differences in B lineage composition: ASCs (1.2% vs 44.1%), germinal center (23.1% vs 0.2%), proliferative (12.6% vs 3.0%), and non-ASCs (63.1% vs 52.7%), respectively. NPs exhibited higher expression (>2-fold) of specific isotypes (IGHE, IGHA1, IGHA2, and IGHG4), and mature plasma genes including SDC1 and XBP1 than tonsils. GO biological processes indicated upregulated NF-κB and downregulated apoptosis pathways in NP ASCs. Conclusions: NP EBI2+ ASCs secreted increased total and anti-dsDNA IgG compared to those from tonsils and had molecular features of mature plasma cell differentiation.
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| Overall design |
Nasal polyp (NP) and control tonsil tissues were obtained from 5 CRSwNP patients and 5 tonsilllar patients accordingly during surgery.
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| Contributor(s) |
Bai J, Kato A, Wechsler JB, Schleimer RP, Tan BK |
| Citation(s) |
39253973 |
| |
| Submission date |
Jun 27, 2024 |
| Last update date |
Oct 09, 2024 |
| Contact name |
Matthew J. Schipma |
| E-mail(s) |
m-schipma@northwestern.edu
|
| Phone |
1-312-503-5324
|
| Organization name |
Northwestern University
|
| Department |
Biochemistry and Molecular Genetics
|
| Street address |
750 N. Lake Shore Drive
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA1129109 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE270953_NP21009_barcodes.tsv.gz |
61.0 Kb |
(ftp)(http) |
TSV |
| GSE270953_NP21009_features.tsv.gz |
325.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_NP21009_matrix.mtx.gz |
64.5 Mb |
(ftp)(http) |
MTX |
| GSE270953_NP21010_barcodes.tsv.gz |
64.2 Kb |
(ftp)(http) |
TSV |
| GSE270953_NP21010_features.tsv.gz |
325.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_NP21010_matrix.mtx.gz |
60.3 Mb |
(ftp)(http) |
MTX |
| GSE270953_NP21040_barcodes.tsv.gz |
71.4 Kb |
(ftp)(http) |
TSV |
| GSE270953_NP21040_features.tsv.gz |
325.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_NP21040_matrix.mtx.gz |
73.6 Mb |
(ftp)(http) |
MTX |
| GSE270953_NP22069_barcodes.tsv.gz |
43.4 Kb |
(ftp)(http) |
TSV |
| GSE270953_NP22069_features.tsv.gz |
325.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_NP22069_matrix.mtx.gz |
31.9 Mb |
(ftp)(http) |
MTX |
| GSE270953_NP22077_barcodes.tsv.gz |
41.8 Kb |
(ftp)(http) |
TSV |
| GSE270953_NP22077_features.tsv.gz |
325.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_NP22077_matrix.mtx.gz |
45.7 Mb |
(ftp)(http) |
MTX |
| GSE270953_TN19056_barcodes.tsv.gz |
16.9 Kb |
(ftp)(http) |
TSV |
| GSE270953_TN19056_features.tsv.gz |
325.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_TN19056_matrix.mtx.gz |
13.5 Mb |
(ftp)(http) |
MTX |
| GSE270953_TN21006_barcodes.tsv.gz |
37.2 Kb |
(ftp)(http) |
TSV |
| GSE270953_TN21006_features.tsv.gz |
325.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_TN21006_matrix.mtx.gz |
50.4 Mb |
(ftp)(http) |
MTX |
| GSE270953_TN21055_barcodes.tsv.gz |
8.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_TN21055_features.tsv.gz |
325.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_TN21055_matrix.mtx.gz |
6.3 Mb |
(ftp)(http) |
MTX |
| GSE270953_TN22071_barcodes.tsv.gz |
23.0 Kb |
(ftp)(http) |
TSV |
| GSE270953_TN22071_features.tsv.gz |
325.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_TN22071_matrix.mtx.gz |
11.8 Mb |
(ftp)(http) |
MTX |
| GSE270953_TN22072_barcodes.tsv.gz |
37.1 Kb |
(ftp)(http) |
TSV |
| GSE270953_TN22072_features.tsv.gz |
325.7 Kb |
(ftp)(http) |
TSV |
| GSE270953_TN22072_matrix.mtx.gz |
51.0 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
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