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| Status |
Public on Nov 04, 2024 |
| Title |
Bacterial single-cell RNA sequencing captures biofilm transcriptional heterogeneity and differential responses to immune pressure |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Biofilm formation is an important mechanism of survival and persistence for many bacterial pathogens. These multicellular communities contain subpopulations of cells that display vast metabolic and transcriptional diversity along with high recalcitrance to antibiotics and host immune defenses. Investigating the complex heterogeneity within biofilm has been hindered by the lack of a sensitive and high-throughput method to assess stochastic transcriptional activity and regulation between bacterial subpopulations, which requires single-cell resolution. We have developed an optimized bacterial single-cell RNA sequencing method, BaSSSh-seq, to study Staphylococcus aureus diversity during biofilm growth and transcriptional adaptations following immune cell exposure.
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| Overall design |
BaSSSh-seq was first applied to compare transcriptional profiles of S. aureus biofilm and planktonic growth. BaSSSh-seq was next applied to S. aureus biofilms following direct co-culture with the major leukocyte infiltrates associated with biofilm infection: macrophages, granulocytic myeloid-derived supressor cells, and neutrophils.
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| Contributor(s) |
Korshoj LE, Kielian T |
| Citation missing |
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| Submission date |
Jun 27, 2024 |
| Last update date |
Nov 04, 2024 |
| Contact name |
Lee Erik Korshoj |
| E-mail(s) |
lee.korshoj@unmc.edu
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| Organization name |
University of Nebraska Medical Center
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| Department |
Pathology, Microbiology, and Immunology
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| Street address |
985900 Nebraska Medical Center
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| City |
Omaha |
| State/province |
NE |
| ZIP/Postal code |
68198 |
| Country |
USA |
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| Platforms (1) |
| GPL28116 |
NextSeq 550 (Staphylococcus aureus) |
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| Samples (2) |
| GSM8366613 |
S. aureus, biofilm and planktonic, BaSSSh-seq 1-4 |
| GSM8366614 |
S. aureus, biofilm-leukocyte co-cultures, BaSSSh-seq 1-4 |
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| Relations |
| BioProject |
PRJNA1129153 |
