 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 01, 2012 |
| Title |
Differential cardiac gene expression in genetically selected turkeys |
| Organism |
Meleagris gallopavo |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of turkey cardiac muscle at 1d post-hatch and 16wk birds of F (growth-selected) and RBC2 (randombred) lines
|
| |
|
| Overall design |
In this study, we compared 1d post-hatch cardiac muscle to 16wk market weight for both the F and RBC2 lines using the 6K Turkey Skeletal Muscle Long Oligo (TSKMLO) microarray developed for transcriptional profiling of turkey muscle as it relates to growth, development, and downstream meat quality. The TSKMLO array also includes a number of unique controls probes for each of 30 randomly chosen genes: 1 scrambled sequence control, 3 “distance” controls that included oligos designed within 500, 501-1000, and 1001-1500 bases from the 3’ of the sequence, and 7 “mismatch” controls including 3, 5, 10, 15, 20, or 25 mismatched bases, totaling 300 control probes. There were 3 biological replicates for each comparison for a total of 12 arrays. Dye assignments were reversed an equal number of times within each comparison to avoid dye bias. Birds were randomly assigned to array, and hybridizations were performed randomly.
|
| |
|
| Contributor(s) |
Mendoza KM, Sporer KR, Strasburg GM, Tempelman RJ, Frame D, Velleman SG, Reed KM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 08, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Kent Reed |
| E-mail(s) |
reedx054@umn.edu
|
| Phone |
612-624-1287
|
| Fax |
612-625-0204
|
| Organization name |
University of Minnesota
|
| Department |
Veterinary and Biomedical Sciences
|
| Street address |
295 ASVM, 1988 Fitch Ave
|
| City |
St. Paul |
| State/province |
MN |
| ZIP/Postal code |
55108 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9788 |
Turkey skeletal muscle long oligonucleotide (TSKMLO) microarray |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA137569 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE27147_RAW.tar |
18.3 Mb |
(http)(custom) |
TAR (of CSV, GPR) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...