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Series GSE27230 Query DataSets for GSE27230
Status Public on Jun 17, 2011
Title Analysis of the impact of cellular miRNA signatures on the profiles of circulating miRNA biomarkers (fractionation data)
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Circulating miRNAs are an emerging class of biomarkers correlating their specific expression patterns to disease states. A considerable proportion of hematopoietically-derived miRNAs are present in plasma with the ability to confound the signature of true circulating miRNA species. We use microarray analysis to catalogue a list of 313 haemotopoetic miRNAs and analyze expression profiles of cell-free miRNAs in individual plasma fractions after calibrating for cellular miRNA signals. Comprehensive global maps of bona fide circulating miRNA species are presented, and inter-individual variability and gender-specific expression is explored in populations of healthy individuals.
Overall design Healthy Caucasian individuals were used to segregate blood into 8 subfractions: white blood cells (WBC)/buffy coat, leukocytes, red blood cells (red blood cells (RBC)), cloudy supernatant (CS), supernatant 1 (S1), supernatant 2 (S2), pellet 1 (P1) and pellet 2 (P2) through differential centrifugation to understand the proportion of cellular miRNAs in each category.
Contributor(s) Duttagupta R, Jiang R, Getts RC, Gollub J, Jones KW
Citation(s) 21698099
Submission date Feb 10, 2011
Last update date May 02, 2017
Contact name Radha Duttagupta
Phone 408-731-5691
Fax 408-731-5463
Organization name Affymetrix
Department Applied Research
Street address 3450 Central Expressway
City Santa Clara
State/province CA
ZIP/Postal code 95051
Country USA
Platforms (1)
GPL8786 [miRNA-1] Affymetrix Multispecies miRNA-1 Array
Samples (43)
GSM673243 Plasma_CS_rep1
GSM673244 Plasma_CS_rep2
GSM673245 Plasma_CS_rep3
This SubSeries is part of SuperSeries:
GSE27256 Impact of cellular miRNAs on the biomarker signatures of circulating miRNAs
BioProject PRJNA141965

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27230_RAW.tar 7.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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