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| Status |
Public on Jun 17, 2011 |
| Title |
Analysis of the impact of cellular miRNA signatures on the profiles of circulating miRNA biomarkers (fractionation data) |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Circulating miRNAs are an emerging class of biomarkers correlating their specific expression patterns to disease states. A considerable proportion of hematopoietically-derived miRNAs are present in plasma with the ability to confound the signature of true circulating miRNA species. We use microarray analysis to catalogue a list of 313 haemotopoetic miRNAs and analyze expression profiles of cell-free miRNAs in individual plasma fractions after calibrating for cellular miRNA signals. Comprehensive global maps of bona fide circulating miRNA species are presented, and inter-individual variability and gender-specific expression is explored in populations of healthy individuals.
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| Overall design |
Healthy Caucasian individuals were used to segregate blood into 8 subfractions: white blood cells (WBC)/buffy coat, leukocytes, red blood cells (red blood cells (RBC)), cloudy supernatant (CS), supernatant 1 (S1), supernatant 2 (S2), pellet 1 (P1) and pellet 2 (P2) through differential centrifugation to understand the proportion of cellular miRNAs in each category.
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| Contributor(s) |
Duttagupta R, Jiang R, Getts RC, Gollub J, Jones KW |
| Citation(s) |
21698099 |
| |
| Submission date |
Feb 10, 2011 |
| Last update date |
May 02, 2017 |
| Contact name |
Radha Duttagupta |
| E-mail(s) |
Radharani_Duttagupta@affymetrix.com
|
| Phone |
408-731-5691
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| Fax |
408-731-5463
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| Organization name |
Affymetrix
|
| Department |
Applied Research
|
| Street address |
3450 Central Expressway
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| City |
Santa Clara |
| State/province |
CA |
| ZIP/Postal code |
95051 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
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| Samples (43)
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| This SubSeries is part of SuperSeries: |
| GSE27256 |
Impact of cellular miRNAs on the biomarker signatures of circulating miRNAs |
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| Relations |
| BioProject |
PRJNA141965 |
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