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| Status |
Public on Jul 31, 2024 |
| Title |
Spatial Transcriptomic Analysis Of Primary And Metastatic Pancreatic Cancers Highlights Tumor Microenvironmental Heterogeneity |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Although the spatial, cellular, and molecular landscapes of resected pancreatic ductal adenocarcinoma (PDAC) are well documented, the characteristics of its metastatic ecology remain elusive. By applying spatially resolved transcriptomics to matched primary and metastatic PDAC samples, we discovered a conserved continuum of fibrotic, metabolic, and immunosuppressive spatial ecotypes across anatomical regions. We observed spatial tumor microenvironment heterogeneity spanning beyond previously appreciated in PDAC. Through comparative analysis, we show the spatial ecotypes exhibit distinct enrichment between primary and metastatic sites, implying adaptability to the local environment for survival and progression. The invasive border ecotype exhibits both protumorigenic and antitumorigenic cell-type enrichment, suggesting a potential immunotherapy target. The ecotype heterogeneity across patients emphasizes the rationale to map individual patient landscapes to develop personalized treatment strategies. Collectively, our findings provide critical insights into metastatic PDAC biology and serve a valuable resource for future therapeutic exploration and molecular investigations.
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| Overall design |
An atlas of Spatial Transcriptomics from a unique cohort of 30 specimens: 10 primary PDAC tumors (PTs), 3 normal pancreata (NPs), 12 matched hepatic metastases (HMs), 5 matched lymph node metastases (LNMs), along with adjacent normal liver and lymph node tissues, totaling 91,496 spot transcriptomes.
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| Web link |
https://www.nature.com/articles/s41588-024-01914-4
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| Contributor(s) |
Khaliq AM, Rajamohan M, Saeed O, Masood A |
| Citation(s) |
39294496 |
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| Submission date |
Jul 16, 2024 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Ashiq Masood |
| E-mail(s) |
atheeq.khaliq@gmail.com
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| Phone |
3313156813
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| Organization name |
IU School Of Medicine
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| Department |
hematology & Oncology
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| Lab |
Masood Lab
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| Street address |
Melvin and Bren Simon Comprehensive Cancer Center, Indiana University School of Medicine
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| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA1136518 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE272362_RAW.tar |
248.2 Mb |
(http)(custom) |
TAR (of CSV, JPG, JSON, PNG) |
SRA Run Selector |
| Raw data are available in SRA |
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