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Series GSE27299 Query DataSets for GSE27299
Status Public on Oct 01, 2011
Title The impact of chronic temperature elevation on the spleen anti-viral transcriptomic response in Atlantic cod (Gadus morhua)
Organism Gadus morhua
Experiment type Expression profiling by array
Summary This experiment was designed to investigate the impacts of non-lethal increases in temperature on the anti-viral transcriptomic response of Atlantic cod. Selected genes identified as differentially expressed between samples injected with pIC but held at different temperatures were validated using QPCR.
 
Overall design This was a common reference design. For the common reference each individual sample analyzed in the microarray experiment contributed with equal amounts of RNA to a pool. Sixty individuals were analyzed representing 6 biological replicates from 10 different experimental groups. Two separate control groups were used. Non-injected groups at the final temperatures (i.e. 10°C and 16°C) were used as prior to injection controls. At each time-point and temperatures there was also a sham-injected time matched control (injected with phosphate buffered saline [PBS]). The time points were 6 and 24 hours post-injection. Immunostimulated fish were injected with a weight-based dose of poly (I:C)(2 µg per g of wet body mass).
 
Contributor(s) Hori TS, Gamperl KA, Booman M, Nash GW, Rise ML
Citation(s) 22928584
Submission date Feb 14, 2011
Last update date Oct 28, 2019
Contact name Tiago Hori
E-mail(s) tshori@mun.ca
Phone 7098644794
Organization name Memorial University of Newfoundland
Department Ocean Sciences Centre
Lab Rise Lab
Street address 0 Marine Lab Rd
City St John's
State/province NL
ZIP/Postal code A1C5S7
Country Canada
 
Platforms (1)
GPL10532 CGP Atlantic cod 20K oligo array v1.0
Samples (60)
GSM675013 Pre-injected fish held at 10C - rep 1
GSM675014 Pre-injected fish held at 10C - rep 2
GSM675015 Pre-injected fish held at 10C - rep 3
Relations
BioProject PRJNA137355

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27299_RAW.tar 642.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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