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| Status |
Public on Jul 25, 2024 |
| Title |
Modulation by NPYR underlies experience-dependent, sexually dimorphic learning |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The evolutionary paths taken by each sex within a given species sometimes diverge, resulting in behavioral differences. Given their distinct needs, the mechanism by which each sex learns from a shared experience is still an open question. Here, we reveal sexual dimorphism in learning: C. elegans males do not learn to avoid the pathogenic bacteria PA14 as efficiently and rapidly as hermaphrodites. Notably, neuronal activity following pathogen exposure was dimorphic: hermaphrodites generate robust representations while males, in line with their behavior, exhibit contrasting representations. Transcriptomic and behavioral analysis revealed that the neuropeptide receptor npr-5, an ortholog of the mammalian NPY receptor, regulates male learning by modulating neuronal activity. Furthermore, we show the dependency of the males’ decision-making on their sexual status and demonstrate the pivotal role of npr-5 as a modulator of incoming sensory cues. Taken together, we portray sex-specific plasticity in behavior toward a shared experience by modulating learning.
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| Overall design |
To understand the whole animal transcriptomics of hermaphrodites and males towards PA14 exposure after 6 hours. We utilized bulk-optimized protocol of Marseq to derive transcriptomic datsets of 4 biological replicates of each sex after 6 hours exposure to OP50 or PA14
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| Contributor(s) |
Peedikayil-Kurien S, Haque R, Gat A, Oren-Suissa M |
| Citation missing |
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| |
| Submission date |
Jul 25, 2024 |
| Last update date |
Jul 25, 2024 |
| Contact name |
Rizwanul Haque |
| E-mail(s) |
rizwanul.haque@weizmann.ac.il
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| Organization name |
Weizmann Institute of Science
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| Department |
Brain Sciences
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| Lab |
Meital Oren-Suissa lab
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| Street address |
Herzl St 234, Rehovot
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| City |
Rehovot |
| State/province |
Israel |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
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| Platforms (1) |
| GPL19757 |
Illumina NextSeq 500 (Caenorhabditis elegans) |
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| Samples (16)
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| GSM8421698 |
biol rep 1 YA Hermaphrodite him-5(e1490) strain of Caenorhabditis elegans exposed to OP50 for 6 hours |
| GSM8421699 |
biol rep 2 YA Hermaphrodite him-5(e1490) strain of Caenorhabditis elegans exposed to OP50 for 6 hours |
| GSM8421700 |
biol rep 3 YA Hermaphrodite him-5(e1490) strain of Caenorhabditis elegans exposed to OP50 for 6 hours |
| GSM8421701 |
biol rep 4 YA Hermaphrodite him-5(e1490) strain of Caenorhabditis elegans exposed to OP50 for 6 hours |
| GSM8421702 |
biol rep 1 YA Male him-5(e1490) strain of Caenorhabditis elegans exposed to OP50 for 6 hours |
| GSM8421703 |
biol rep 2 YA Male him-5(e1490) strain of Caenorhabditis elegans exposed to OP50 for 6 hours |
| GSM8421704 |
biol rep 3 YA Male him-5(e1490) strain of Caenorhabditis elegans exposed to OP50 for 6 hours |
| GSM8421705 |
biol rep 4 YA Male him-5(e1490) strain of Caenorhabditis elegans exposed to OP50 for 6 hours |
| GSM8421706 |
biol rep 1 YA Hermaphrodite him-5(e1490) strain of Caenorhabditis elegans exposed to PA14 for 6 hours |
| GSM8421707 |
biol rep 2 YA Hermaphrodite him-5(e1490) strain of Caenorhabditis elegans exposed to PA14 for 6 hours |
| GSM8421708 |
biol rep 3 YA Hermaphrodite him-5(e1490) strain of Caenorhabditis elegans exposed to PA14 for 6 hours |
| GSM8421709 |
biol rep 4 YA Hermaphrodite him-5(e1490) strain of Caenorhabditis elegans exposed to PA14 for 6 hours |
| GSM8421710 |
biol rep 1 YA Male him-5(e1490) strain of Caenorhabditis elegans exposed to PA14 for 6 hours |
| GSM8421711 |
biol rep 2 YA Male him-5(e1490) strain of Caenorhabditis elegans exposed to PA14 for 6 hours |
| GSM8421712 |
biol rep 3 YA Male him-5(e1490) strain of Caenorhabditis elegans exposed to PA14 for 6 hours |
| GSM8421713 |
biol rep 4 YA Male him-5(e1490) strain of Caenorhabditis elegans exposed to PA14 for 6 hours |
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| Relations |
| BioProject |
PRJNA1140164 |
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