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| Status |
Public on Aug 04, 2024 |
| Title |
Post-prandial cardiac hypertrophy is sustained by mechanics, epigenetic, and metabolic reprogramming in pythons [RNA-Seq] |
| Organism |
Python regius |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Constricting pythons, known for their ability to consume infrequent, massive meals, exhibit rapid and reversible cardiac hypertrophy following feeding. Our primary goal was to investigate how python hearts achieve this adaptive response after feeding. Isolated myofibrils increased force after feeding without changes in sarcomere ultrastructure and without increasing energy cost. Ca2+ transients were prolonged after feeding with no changes in myofibril Ca2+ sensitivity. Feeding reduced titin-based tension, resulting in decreased cardiac tissue stiffness. Feeding also reduced the activity of sirtuins, a metabolically-linked class of histone deacetylases, and increased chromatin accessibility. A transcription factor enrichment analysis on transposase-accessible chromatin with sequencing (ATAC-Seq) revealed the prominent role of transcription factors YY1 and NRF1 in post-feeding cardiac adaptation. Gene expression was also changed in favor of translation and metabolism. Finally, metabolomics analysis and ATP production assay demonstrated that cardiac adaptation after feeding not only increased energy demand but also energy production. These findings have broader implications for our understanding of cardiac adaptation across species and hold promise for the development of innovative approaches to address cardiovascular diseases.
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| Overall design |
To determine the gene expression profile of post-prandial python hearts, the hearts of fasted and 24 post fed Python regius were collected for RNA-sequencing analysis.
Three fasted and three fed pythons were included in the study.
Gene expression analysis was performed between fasted and 24 hour post fed python hearts.
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| Contributor(s) |
Crocini C, Woulfe KC, Ozeroff CD, Perni S, Cardiello J, Walker CJ, Anseth KS, Allen MA, Leinwand LA |
| Citation(s) |
39159386 |
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| Submission date |
Jul 31, 2024 |
| Last update date |
Oct 09, 2024 |
| Contact name |
Mary Ann Allen |
| E-mail(s) |
mary.a.allen@colorado.edu
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| Phone |
608-209-6921
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| Organization name |
University of Colorado
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| Department |
BioFrontiers
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| Lab |
Allen
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| Street address |
UCB 596 JSCBB
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| City |
Boulder |
| State/province |
Co |
| ZIP/Postal code |
80309 |
| Country |
USA |
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| Platforms (1) |
| GPL27701 |
Illumina NextSeq 500 (Python regius) |
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| Samples (6)
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| GSM8432266 |
Python Regius, Heart, 28 days fasted replicate 1 |
| GSM8432267 |
Python Regius, Heart, 28 days fasted replicate 2 |
| GSM8432268 |
Python Regius, Heart, 28 days fasted replicate 3 |
| GSM8432269 |
Python Regius, Heart, 24 hours post feeding replicate 1 |
| GSM8432270 |
Python Regius, Heart, 24 hours post feeding replicate 2 |
| GSM8432271 |
Python Regius, Heart, 24 hours post feeding replicate 3 |
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| Relations |
| BioProject |
PRJNA1142400 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE273554_2021_01_14_deseq_res_for_gseaTranscriptIDs.normcounts.txt.gz |
722.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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