|
| Status |
Public on Dec 31, 2011 |
| Title |
Burkholderia pseudomallei transcriptional adaptation in macrophage |
| Platform organism |
Burkholderia pseudomallei K96243 |
| Sample organism |
Burkholderia pseudomallei |
| Experiment type |
Expression profiling by array
|
| Summary |
This study reports the expression profile of intracellular B. pseudomallei following infection of human macrophage-like U937 cells. The transcriptome of intracellular B. pseudomallei harvested from macrophage cells over an infection period of 6 hr (1, 2, 4 and 6 hr post-infection) were compared to in vitro grown bacteria to identify genes whose expression is altered in response to intracellular growth.
|
| |
|
| Overall design |
The experimental design of this study involved the total RNA from intracellular B. pseudomallei harvested from infected U937 cells at 4 selected time points with 3 biological replicates each. The microarray data from each time point was compared relative to in vitro grown bacteria in cell culture medium.
|
| |
|
| Contributor(s) |
Chieng S, Nathan S |
| Citation(s) |
22823543 |
| |
| Submission date |
Feb 28, 2011 |
| Last update date |
Dec 30, 2013 |
| Contact name |
Sheila Nathan |
| E-mail(s) |
snathan2005@yahoo.co.uk
|
| Phone |
+60389267446
|
| Fax |
+60389252698
|
| Organization name |
Universiti Kebangsaan Malaysia
|
| Department |
Faculty of Science & Technology
|
| Lab |
Block 5B
|
| Street address |
Jalan Universiti
|
| City |
Bangi |
| State/province |
Selangor |
| ZIP/Postal code |
43600 |
| Country |
Malaysia |
| |
|
| Platforms (1) |
| GPL13233 |
Agilent-019078 Custom Burkholderia pseudomallei 8X15K microarray |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA138393 |
Less...
More...