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Status |
Public on Jun 26, 2012 |
Title |
Profile of small RNA transcriptome during cortical development in the Rat |
Organism |
Rattus norvegicus |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
Summary |
Small non-coding RNAs play important roles in regulating gene expression during development and disease. However, a comprehensive analysis of small RNAs in developing brain is largely lacking. By using next-generation sequencing technique, we carried out a systematic analysis of small RNA transcriptome of rat neocortex from early developmental stage till adult. Our results revealed an extraordinary degree of stage-specific profiling of miRNA, and other classes of small RNAs including snoRNA, rasRNA, scRNA and unexpectedly piRNA, which was considered to be solely expressed in germline tissues. Developmentally regulated miRNA editing was also observed. This study further showed the effectiveness of the high through-put sequencing in genome-wide analysis of small RNAs. The dataset described here will greatly contribute to our understanding of the divergence, variation, and function of small RNAs and may be a valuable resource in clarifying new regulatory mechanisms of cortical development.
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Overall design |
Identification and quantification of small RNA: forebrain and cortex from E10 to P28
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Contributor(s) |
Xiaobing Y, Maojin Y, Gang C |
Citation(s) |
22691069 |
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Submission date |
Feb 28, 2011 |
Last update date |
Jun 11, 2013 |
Contact name |
Maojin Yao |
E-mail(s) |
mjyao@ion.ac.cn
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Organization name |
Institute of Neurosciecne
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Department |
Neural circuit development
|
Street address |
320# Yueyang Road
|
City |
Shanghai |
ZIP/Postal code |
200031 |
Country |
China |
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Platforms (1) |
GPL13235 |
Illumina genome Analyzer II (Rattus norvegicus) |
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Samples (18)
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GSM683700 |
Forebrain_Embryonic day 10_Technical replicate_1 |
GSM683701 |
Forebrain_Embryonic day 10_Technical replicate_2 |
GSM683702 |
Forebrain_Embryonic day 13_Technical replicate_1 |
GSM683703 |
Forebrain_Embryonic day 13_Technical replicate_2 |
GSM683704 |
Somatosensory cortex_Embryonic day 17_Technical replicate_1 |
GSM683705 |
Somatosensory cortex_Embryonic day 17_Technical replicate_2 |
GSM683706 |
Somatosensory cortex_Postnatal day 0_biological replicate_1_Technical replicate_1 |
GSM683707 |
Somatosensory cortex_Postnatal day 0_biological replicate_1_Technical replicate_2 |
GSM683708 |
Somatosensory cortex_Postnatal day 0_biological replicate_2_Technical replicate_1 |
GSM683709 |
Somatosensory cortex_Postnatal day 0_biological replicate_2_Technical replicate_2 |
GSM683710 |
Somatosensory cortex_Postnatal day 3_Technical replicate_1 |
GSM683711 |
Somatosensory cortex_Postnatal day 3_Technical replicate_2 |
GSM683712 |
Somatosensory cortex_Postnatal day 7_Technical replicate_1 |
GSM683713 |
Somatosensory cortex_Postnatal day 7_Technical replicate_2 |
GSM683714 |
Somatosensory cortex_Postnatal day 14_Technical replicate_1 |
GSM683715 |
Somatosensory cortex_Postnatal day 14_Technical replicate_2 |
GSM683716 |
Somatosensory cortex_Postnatal day 28_Technical replicate_1 |
GSM683717 |
Somatosensory cortex_Postnatal day 28_Technical replicate_2 |
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Relations |
BioProject |
PRJNA138383 |