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| Status |
Public on Sep 02, 2024 |
| Title |
Investigating Subpopulation Dynamics in Clonal CHO-K1 Cells with Single-Cell RNA Sequencing |
| Organism |
Cricetulus griseus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CHO cells have primarily been studied in bulk, assuming that these bulk samples are identical because of genetic clonality across the sample. In this study, we performed single-cell RNA sequencing on two clonal CHO-K1 cell populations with different stability phenotypes over a 90 day culture period.
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| Overall design |
Two Chinese Hamster Ovary were cultured for 30 population doublings in different culture conditions, with samples gathered before the first and final passages. Banked samples were sequenced with scRNA sequencing.
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Authors state "due to company restrictions (our sample source) they do not want us to deposit the raw FASTQ (Level 1) but will allow the counts matrix to be deposited".
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| Contributor(s) |
Morina L, Cao HC, Chen S, Kumar S, McFarland KS, Majewska NI, Betenbaugh MJ, Timp W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 28, 2024 |
| Last update date |
Sep 02, 2024 |
| Contact name |
Luke B Morina |
| Organization name |
Johns Hopkins University
|
| Street address |
3400 N Charles St; CLARK HALL Rm118a
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21218 |
| Country |
USA |
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| Platforms (1) |
| GPL27425 |
Illumina NovaSeq 6000 (Cricetulus griseus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1153607 |
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