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| Status |
Public on Mar 02, 2012 |
| Title |
Transcriptional profiling of Aspergillus fumigatus in response to RBL-2H3 mast cells |
| Platform organism |
Aspergillus fumigatus Af293 |
| Sample organism |
Aspergillus fumigatus |
| Experiment type |
Expression profiling by array
|
| Summary |
In patients with chronic pulmonary disease colonization with the mold Aspergillus fumigatus is associated with declining pulmonary function and obstructive airway disease. One potential effector of this inflammatory response is the pulmonary mast cell. In vitro studies have demonstrated that A. fumigatus contact induces IgE-independent mast cell degranulation. Conversely, the Aspergillus secondary metabolite gliotoxin has been shown to suppress mast cell activation. These contradictory results emphasize the need for a better understanding of the interactions between A. fumigatus and mast cells. Thus, the objective of this work was to identify A. fumigatus genes that are differentially regulated upon exposure to mast cells. Transcriptional profiling experiments indicated that, in addition to genes encoding for iron acquisition systems, allergens and putative virulence factors, genes from the gliotoxin biosynthesis cluster were significantly down-regulated upon exposure to mast cells. Globally, the results from this study provide insight into the A. fumigatus response to mast cells and suggest that one mechanism by which the host may circumvent the effects of gliotoxin is via the suppression of fungal gliotoxin synthesis by mast cells.
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| Overall design |
Aspergillus fumigatus hyphae were exposed either to RBL-2H3 mast cells or culture medium for 1.5hr or 3hrs. Hybridizations were performed with biological replicates and flip-dye pairs.
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| Contributor(s) |
Sheppard DC, Vanier G, Chen D, Nierman WC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 01, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Natalie Fedorova |
| E-mail(s) |
natalief@jcvi.org
|
| Phone |
301-795-7756
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| Fax |
301-838-0208
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| Organization name |
JCVI
|
| Department |
Infectious Disease
|
| Street address |
9704 Medical Center Dr
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
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| Platforms (1) |
| GPL10341 |
JCVI Aspergillus fumigatus AF293 29.9K amplicon array |
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| Samples (6)
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| GSM684487 |
Af293 vs Af293+Mast cells 1.5hr Replicate 1 |
| GSM684488 |
Af293 vs Af293+Mast cells 1.5hr Replicate 2 |
| GSM684489 |
Af293 vs Af293+Mast cells 1.5hr Replicate 3 |
| GSM684490 |
Af293 vs Af293+Mast cells 3hrs Replicate 1 |
| GSM684491 |
Af293 vs Af293+Mast cells 3hrs Replicate 2 |
| GSM684492 |
Af293 vs Af293+Mast cells 3hrs Replicate 3 |
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| Relations |
| BioProject |
PRJNA138257 |
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