|
| Status |
Public on Sep 01, 2024 |
| Title |
CRISPRi/a screen of doxorubicin induced cardiotoxicity |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Druggable genome of CRISPRi/a sgRNA libraries for druggable genes protecting doxorubicin induced cardiomyocyte toxicity using human induced pluripotent stem cell (iPSC) derived cardiomyocytes.
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| |
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| Overall design |
Vehicle (DMSO) and doxorubicin treated iPSC differentiated cardiomyocytes (iCMs). iCMs were transfected with sgRNA libraries and CRISPRi (dCas9-KRAB) or CRISPRa (dCas9-VPR). Two biological replicates were used in both CRISPRi and CRISPRa screens.
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| |
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| Contributor(s) |
Liu C, Shen M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 01, 2024 |
| Last update date |
Sep 01, 2024 |
| Contact name |
Chun Liu |
| E-mail(s) |
liuchun@stanford.edu
|
| Organization name |
stanford
|
| Street address |
1291 Welch Road BMI building, 3200-CVI Rm
|
| City |
palo alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1155211 |
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