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Series GSE27630 Query DataSets for GSE27630
Status Public on Feb 05, 2013
Title The transcription factor Otx2 regulates choroid plexus development and function
Organism Mus musculus
Experiment type Expression profiling by array
Summary The choroid plexuses (ChPs) are the main regulators of cerebrospinal fluid (CSF) composition and thereby also control the composition of a principal source of signaling molecules that is in direct contact with neural stem cells in the developing brain. The regulators of ChP development mediating the acquisition of a fate that differs from the neighboring neuroepithelial cells are poorly understood. Here, we demonstrate in mice a crucial role for the transcription factor Otx2 in the development and maintenance of ChP cells. Deletion of Otx2 by the Otx2-CreERT2 driver line at E9 resulted in a lack of all ChPs, whereas deletion by the Gdf7-Cre driver line affected predominately the hindbrain ChP, which was reduced in size, primarily owing to an increase in apoptosis upon Otx2 deletion. Strikingly, Otx2 was still required for the maintenance of hindbrain ChP cells at later stages when Otx2 deletion was induced at E15, demonstrating a central role of Otx2 in ChP development and maintenance. Moreover, the predominant defects in the hindbrain ChP mediated by Gdf7-Cre deletion of Otx2 revealed its key role in regulating early CSF composition, which was altered in protein content, including the levels of Wnt4 and the Wnt modulator Tgm2. Accordingly, proliferation and Wnt signaling levels were increased in the distant cerebral cortex, suggesting a role of the hindbrain ChP in regulating CSF composition, including key signaling molecules. Thus, Otx2 acts as a master regulator of ChP development, thereby influencing one of the principal sources of signaling in the developing brain, the CSF.
 
Overall design We performed gene expression microarray analysis of fourth ventricular choroid plexus tissue from Otx2 k.o. mice compared to wildtype mice from the same litters.
 
Contributor(s) Johansson P, Irmler M, Acampora D, Beckers J, Simeone A, Goetz M
Citation(s) 23364326
Submission date Mar 02, 2011
Last update date Feb 11, 2019
Contact name Johannes Beckers
E-mail(s) johannes.beckers@helmholtz-munich.de
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM684727 Fourth ventricular choroid plexus from Otx2ko mice at E13, biological replicate 1
GSM684728 Fourth ventricular choroid plexus from Otx2ko mice at E13, biological replicate 2
GSM684729 Fourth ventricular choroid plexus from Otx2ko mice at E13, biological replicate 3
Relations
BioProject PRJNA138529

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27630_RAW.tar 28.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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