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| Status |
Public on Oct 24, 2024 |
| Title |
Bone-protective effects of neutralizing angiopoietin-like protein 4 monoclonal antibody in rheumatoid arthritis |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Despite recent advances, rheumatoid arthritis (RA) patients remain refractory to therapy. Dysregulated overproduction of angiopoietin-like protein 4 (ANGPTL4) is thought to contribute to the disease development. ANGPTL4 was initially identified as a regulator of lipid metabolism, which is hydrolyzed to N-terminal and C-terminal (cANGPTL4) fragments in vivo. cANGPTL4 is involved in several non-lipid-related processes, including angiogenesis and inflammation. This study revealed that the level of ANGPTL4 was markedly elevated in the sera and synovial tissues from patients with RA versus controls. The administration of a neutralizing antibody against cANGPTL4 (anti-cANGPTL4 Ab) resulted in the inhibition of inflammatory processes and bone loss in animal models of collagen-induced arthritis and adjuvant-induced arthritis (AIA). Transcriptomic and proteomic profiling of synovial tissues from an AIA model indicated that the anti-cANGPTL4 Ab inhibited fibroblast-like synoviocyte (FLS) immigration and inflammatory-induced osteoclastogenesis. Mechanistically, the anti-cANGPTL4 Ab has been shown to inhibit TNF-α-induced inflammatory cascades in RA-FLS through the sirtuin 1/nuclear factor-κB signaling pathway. Moreover, the anti-cANGPTL4 Ab was found to block FLS invasion- and immigration-induced osteoclast activation. Collectively, these findings identify ANGPTL4 as a prospective biomarker for the diagnosis of RA, and targeting cANGPTL4 should represent a potential therapeutic strategy.
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| Overall design |
Comparative gene expression profiling analysis on the RNA-seq data of rat synovial tissues of adjuvant-induced arthritis rat model (CON, AIA and Ab, n=5).
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| Web link |
https://doi.org/10.1016/j.ymthe.2024.09.031
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| |
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| Contributor(s) |
Cheng W, Li L, Zhang P, Ke L |
| Citation(s) |
39367607 |
| |
| Submission date |
Sep 06, 2024 |
| Last update date |
Oct 24, 2024 |
| Contact name |
Wenxiang Cheng |
| E-mail(s) |
wenxiang.cheng@gmail.com
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| Phone |
8613316801693
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| Organization name |
Shenzhen Institutes Of Advanced Technology, Chinese Academy Of Sciences,
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| Street address |
1068
|
| City |
Shenzhen |
| State/province |
Guangdong |
| ZIP/Postal code |
518055 |
| Country |
China |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA1157843 |
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