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| Status |
Public on Sep 11, 2024 |
| Title |
FGF8-mediated gene regulation affects regional identity in human cerebral organoids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Single cell RNA sequencing
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| Overall design |
Human cortical organoids (day 69 of differentiation); 2 control batches (3 organoids each) and 2 FGF8-treated batches (3 organoids each). 17,400 cells per channel (corresponding to an estimated recovery of 10,000 cells per channel) were loaded onto a Chromium Single Cell 3′ Chip (10x Genomics, PN 2000177). scRNA-seq libraries were prepared with the Chromium Single Cell 3′ Library & Gel Bead Kit v.2 (10x Genomics, PN-120237). Libraries were sequenced using Illumina HiSeq 4000 system in Paired-End mode with 100 or 28 bases for read 1 and 100 bases for read 2, following Illumina's instructions. Image analysis and base calling were performed using RTA version 2.7.2 and Cell Ranger version 3.0.2.
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| Contributor(s) |
Bertacchi M, Jung M, Studer M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 06, 2024 |
| Last update date |
Sep 11, 2024 |
| Contact name |
Michele Bertacchi |
| E-mail(s) |
mbertacchi@unice.fr, Michele.BERTACCHI@univ-cotedazur.fr
|
| Phone |
+33489150721
|
| Organization name |
iBV - Institut de Biologie Valrose
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| Lab |
Studer Lab
|
| Street address |
28, avenue Valrose
|
| City |
Nice |
| State/province |
Provence Alpes Côte d Azur |
| ZIP/Postal code |
06108 |
| Country |
France |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1157844 |
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