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| Status |
Public on Oct 01, 2024 |
| Title |
Terminal differentiation and persistence of effector regulatory T cells essential for the prevention of intestinal inflammation [scMultiomics] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Regulatory T (Treg) cells represent a specialized CD4+ T cell lineage with essential anti-inflammatory functions. Recent studies of the adaptations of Treg cells to non-lymphoid tissues which enable their specialized immunosuppressive and tissue supportive functions raise questions about the underlying mechanisms of these adaptations and whether they represent stable differentiation or reversible activation states. Using novel genetic tools, we characterized the transcriptional programs of distinct colonic effector Treg cell types. We found that attenuated T cell receptor (TCR) signaling and acquisition of substantial TCR independent functionality appears to facilitate the terminal differentiation of a population of colonic effector Treg cells distinguished by stable expression of immunomodulatory cytokine interleukin-10 (IL-10). Functional studies revealed that this subset of effector Treg cells, but not their expression of IL-10, was indispensable for preventing colitis. These findings suggest core features of terminal differentiation of effector Treg cells in non-lymphoid tissues and their function therein.
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| Overall design |
We performed scRNA-seq on total Treg cells from large intestine and small intestine in order to identify potential subpopulations within each as well as to gain insight into relationships between and potential differentiation trajectories of Il10 expressing and non Il10 expressing Treg cell subsets within each tissue.
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| Contributor(s) |
Dikiy S, Ghelani AP, Levine AG, Martis S, Giovanelli P, Wang Z, Beroshvili G, Pritykin Y, Krishna C, Huang X, Glasner A, Greenbaum BD, Leslie CS, Rudensky AY |
| Citation missing |
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| Submission date |
Oct 01, 2024 |
| Last update date |
Oct 02, 2024 |
| Contact name |
Stanislav Dikiy |
| E-mail(s) |
sdikiy@scripps.edu
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| Organization name |
Scripps Research
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| Department |
Immunology
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| Lab |
Hang
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| Street address |
10550 North Torrey Pines Road
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE207969 |
Terminal differentiation and persistence of effector regulatory T cells essential for the prevention of intestinal inflammation |
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| Relations |
| BioProject |
PRJNA1167747 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE278537_RAW.tar |
275.9 Mb |
(http)(custom) |
TAR (of CSV, H5AD, TAR, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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