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| Status |
Public on Nov 01, 2021 |
| Title |
An Antagonist of Toll-like Receptor 7 and 9 lowers cholesterol and reduces atherosclerosis in hyperlipidemic mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The impact of a dual TLR7 and TLR9 antagonist on hypercholesteremia and atherosclerosis was evaluated in hyperlipidemic mice. Treatment with antagonist induced a dose dependent reduction of total cholesterol (TC) and LDL-cholesterol. Changes in Adiponectin, Leptin and free fatty acids levels were observed. Plaque formation was inhibited in ApoE-/- and LDL-R-/- mice fed high fat diet and treated with the antagonist. Hepatic and renal steatosis was reduced by the treatment. Induction of IL-10 expression was inversely correlated with TC levels. The antagonist induced decrease in hepatic IKKα protein level and enhancement of Akt and Gsk3β phosphorylation. Treatment of C57BL/6 mice caused increase in hepatic LXR, PPARγ and ABCG1 expression and in stool content of TC. Antagonist treatment resulted in altered expression of several genes and pathways related to immune system, cholesterol, fatty acid and glucose metabolism. Data obtained suggest involvement of TLR7 and TLR9 in diet induced hyperlipidemia and atherosclerosis and demonstrate therapeutic potential of TLR7/9 antagonist.
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| Overall design |
Total RNA isolated from C57BL/6 HFD and ApoE-/- HFD mice treated with antagonist compared to untreated mice
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| Contributor(s) |
Zhu F, Jiang W, Uva P, Mentzen W, Reardon M, Yu D, Kandimalla ER, La Monica N, Agrawal S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 23, 2011 |
| Last update date |
Nov 01, 2021 |
| Contact name |
Paolo Uva |
| Organization name |
IRCCS Istituto Giannina Gaslini
|
| Lab |
Clinical Bioinformatics
|
| Street address |
Via Gerolamo Gaslini, 5
|
| City |
Genoa |
| ZIP/Postal code |
16147 |
| Country |
Italy |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA139849 |
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