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Status |
Public on Mar 25, 2011 |
Title |
Topography-dependent gene expression in migratory glioma cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
The goal of this experiment was to compare the gene expression of glioma cells migrating on substrates of different topographies and to determine a migration-associated gene profile. Human malignant glioma U251MG cells were cultured on highly-aligned versus randomly-oriented electrospun nanofibers of poly-caprolactone. Cells migrated actively on aligned nanofibers but motility was very restricted on randomly-oriented fibers. Results of gene expression profiling indicated upregulation of a JAK/STAT signature in actively migrating glioma cells.
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Overall design |
Total RNA was extracted from 3 independent cultures of U251 glioma cells cultured on aligned versus random nanofibers and applied to Affymetrix HG-U133 plus 2 chips. Analysis of gene expression was used to determine a gene-signature correlated with different substrate topography
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Contributor(s) |
Viapiano MS, Lawler SE, Liyanarachchi S |
Citation missing |
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Submission date |
Mar 24, 2011 |
Last update date |
Mar 25, 2019 |
Contact name |
Mariano Sebastian Viapiano |
E-mail(s) |
viapiano.1@osu.edu
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Phone |
614-292-4362
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Fax |
614-292-5379
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URL |
http://www.viapianolab.org
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Organization name |
The Ohio State University
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Department |
Neurological Surgery
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Street address |
1060 Carmack Rd
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City |
Columbus |
State/province |
OH |
ZIP/Postal code |
43210 |
Country |
USA |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (6)
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Relations |
BioProject |
PRJNA139515 |