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| Status |
Public on Mar 26, 2011 |
| Title |
Genes differentially expressed in wild-type Medicago truncatula plants during nodulation |
| Platform organisms |
Sinorhizobium meliloti; Medicago truncatula |
| Sample organism |
Medicago truncatula |
| Experiment type |
Expression profiling by array
|
| Summary |
We used an Affymetrix oligonucleotide microarray consisting of 9,935 tentative consensus (TC)sequences, which are based on cDNA libraries (Mitra et al., 2004 [PMID:15220482]). We examined gene expression of wild-type M. truncatula plants after inoculation with wild-type S. meliloti at 1 d, 4 d, 7d, 14 d, and 21 d. We chose these time points because they span the range of development of the Rhizobium-legume symbiosis, from initiation of the interaction through nitrogen fixation. The gene expression data for the 1-day time point (Mitra et al., 2004 [PMID 15220482]) and the other time points was previously published (Starker el al., 2006 [PMID 16407449]).
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| Overall design |
Three or five independent biological replicates were performed at each time point (1, 4, 7, 14 and 21 days after treatment) and each treatment (buffer and wild-type bacteria).
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| Contributor(s) |
Starker CG, Mitra RM, Rightmyer AP |
| Citation(s) |
15220482, 16407449 |
| |
| Submission date |
Mar 24, 2011 |
| Last update date |
Feb 17, 2015 |
| Contact name |
Adriana Parra Rightmyer |
| E-mail(s) |
Adriana@ArtCubeSoftware.com
|
| Organization name |
Stanford University
|
| Department |
Biology
|
| Lab |
Sharon R. Long
|
| Street address |
371 Serra Mall
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL9757 |
[stSmel_Mtra] SRL_SU Sinorhizobium meliloti/Medicago truncatula dual-genome SymbiosisChip, Affymetrix part number 510699 |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE28174 |
Expression data from Medicago truncatula roots treated with S. meliloti wild-type or exoA mutant bacteria, or auxin transport inhibitors |
|
| Relations |
| BioProject |
PRJNA143149 |
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