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| Status |
Public on Jun 21, 2005 |
| Title |
Identification of PM-localized proteins |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Other
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| Summary |
In order to assess the quality of alleged PM identifications from Arabidopsis, PM-enriched fractions were compared to PM-depleted fractions using 18O isotopic labeling and mass spectrometry. The two samples submitted are biological replicates.
Keywords: Protein Localization via MS
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| Overall design |
Two biological replicates were analyzed in order to assess sample complexity. PM-derived vesicles were digested in 18O water and compared to PM-depleted fractions digested in 16O (natural abundance) water. Relative ion intensities were then used to calculate a heavy/lite ratio with proteins showing enrichment in the upper phase having a ratio greater than 1 or 0 on a log2 scale.
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| Contributor(s) |
Nelson CJ, Hegeman AD, Harms AC, Sussman MR |
| Citation missing |
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| |
| Submission date |
Jun 21, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Michael R Sussman |
| E-mail(s) |
msussman@wisc.edu
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| Phone |
608-262-8608
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| Fax |
608-262-6748
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| Organization name |
University of Wisconsin
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| Department |
Biotechnology Center
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| Lab |
Sussman
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| Street address |
425 Henry Mall
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| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL2543 |
18O Isotopic Labeling of Plasma Membrane |
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| Samples (2) |
| GSM61946 |
Plasma membrane from Arabidopsis collected from two-phase partitioning (Sample 1) |
| GSM61947 |
Plasma membrane from Arabidopsis collected from two-phase partitioning (Sample 2) |
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| Relations |
| BioProject |
PRJNA92457 |
