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Series GSE2830 Query DataSets for GSE2830
Status Public on Jun 21, 2005
Title Identification of PM-localized proteins
Organism Arabidopsis thaliana
Experiment type Other
Summary In order to assess the quality of alleged PM identifications from Arabidopsis, PM-enriched fractions were compared to PM-depleted fractions using 18O isotopic labeling and mass spectrometry. The two samples submitted are biological replicates.
Keywords: Protein Localization via MS
Overall design Two biological replicates were analyzed in order to assess sample complexity. PM-derived vesicles were digested in 18O water and compared to PM-depleted fractions digested in 16O (natural abundance) water. Relative ion intensities were then used to calculate a heavy/lite ratio with proteins showing enrichment in the upper phase having a ratio greater than 1 or 0 on a log2 scale.
Contributor(s) Nelson CJ, Hegeman AD, Harms AC, Sussman MR
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Submission date Jun 21, 2005
Last update date Mar 16, 2012
Contact name Michael R Sussman
Phone 608-262-8608
Fax 608-262-6748
Organization name University of Wisconsin
Department Biotechnology Center
Lab Sussman
Street address 425 Henry Mall
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
Platforms (1)
GPL2543 18O Isotopic Labeling of Plasma Membrane
Samples (2)
GSM61946 Plasma membrane from Arabidopsis collected from two-phase partitioning (Sample 1)
GSM61947 Plasma membrane from Arabidopsis collected from two-phase partitioning (Sample 2)
BioProject PRJNA92457

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