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Series GSE2836 Query DataSets for GSE2836
Status Public on Jun 25, 2005
Title Hypoxia response
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary The goal of the study was to identify hypoxia-induced gene expression changes in C. elegans and to determine which of these responses to hypoxia were regulated by hif-1. Towards these aims, we analyzed mRNA expression patterns in synchronized populations of wild-type worms that were cultured in standard lab conditions (normoxia) or in hypoxia. We also assayed mRNA from two mutant strains: (i) animals carrying the strong loss-of-function mutation in hif-1 and (ii) C. elegans that carry a deletion in vhl-1 and express the HIF-1 protein at constitutively high levels.
In the microarray experiments, 3 strains were assayed: wild type N2, hif-1 (ia04), and vhl-1 (ok161). Worms were incubated for 4 hours in 21% oxygen or 0.1% oxygen at 210C. Animals were quickly harvested in ice cold M9 buffer, and poly (A) RNA was isolated using established procedures. No more than 3 minutes elapsed between the removal of plates from the hypoxic chamber and the addition of Trizol. RNA was isolated from three independent experiments for each experimental condition (3 genotypes; normoxia vs hypoxia). mRNAs were hybridized to whole-genome microarrays which contained probes for 17,817 predicted genes (94% of the genome).
Ch1 in the array represents the data for the worms growed under hypoxia; and Ch2 in the array reprents the data for the worms growed under normoxia.
Keywords: Biological Replicates
 
Overall design Set of arrays that are part of repeated experiments
 
Contributor(s) Shen C
Citation(s) 15781453
Submission date Jun 24, 2005
Last update date Mar 16, 2012
Contact name Min Jiang
E-mail(s) mjiang@cmgm.stanford.edu
Phone 650-725-7612
Fax 650-725-7739
Organization name Stanford University
Street address -
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL2569 Caenorhabditis elegans printU
Samples (9)
GSM62231 wt 1N vs. wt 1H
GSM62232 vhl-1 1N vs. vhl-1 1H
GSM62233 vhl-1 2N vs. vhl-1 2H
Relations
BioProject PRJNA92469

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Supplementary data files not provided

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