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| Status |
Public on Apr 01, 2015 |
| Title |
8-miR cluster deletion affects mef2 levels in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Loss of nautilus (MyoD) gene function results in a variable phenotype affecting muscle formation in embryos and larvae, larval movement, pupal eclosion, egg deposition, adult mobility and survival. 8-miR cluster deletion disrupts muscle formation in the embryo while affecting protein production from the nautilus, dMef2 , regulators of the muscle transcriptional network. We propose the complex phenotype in the nautilus null is due to the disruption of the regulatory interactions provided by the 8-miR cluster. The results demonstrate that nautilus is an integral regulator of the miRNA circuitry buffering the transcriptional network directing muscle development.
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| Overall design |
Two-condition experiment, wild type (w1118) vs. mutant (8-miR cluster null). Biological replicates: 3 wild type, 3 mutants, independently isolated. One of the biological replicate was dye swaped to avoid dye bias.
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| Contributor(s) |
Ravulapalli A, Qin W, Paterson B |
| Citation missing |
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| |
| Submission date |
Apr 13, 2011 |
| Last update date |
Apr 01, 2015 |
| Contact name |
Anandarao Ravulapalli |
| E-mail(s) |
ravulapa@mail.nih.gov
|
| Phone |
301-594-3314
|
| Organization name |
NCI
|
| Department |
LBMB
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
maryland |
| ZIP/Postal code |
20852 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7300 |
Agilent-018972 D. melanogaster (FruitFly) Oligo Microarray 4x44K G2519F (Probe Name version) |
|
| Samples (4)
|
| GSM707921 |
8-miR cltr null replicate 1 vs. w1118 replicate 1 |
| GSM707922 |
8-miR cltr null replicate 2 vs. w1118 replicate 2 |
| GSM707923 |
8-miR cltr null replicate 3 vs. w1118 replicate 3 |
| GSM707924 |
w1118 replicate 1 vs 8-miR cltr null replicate 1 |
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| This SubSeries is part of SuperSeries: |
| GSE28614 |
Nautilus and muscle development |
|
| Relations |
| BioProject |
PRJNA143007 |
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