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| Status |
Public on May 09, 2014 |
| Title |
Transcriptional profiles of macrophages in resolving inflammation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have performed a comprehensive transcriptional analysis of specific monocyte and macrophage (MØ) subsets during an acute self-resolving inflammatory insult. Following initial induction of acute inflammation, tissue resident (Resident) MØ are rapidly ‘cleared’ from the inflammatory foci, only becoming recoverable as inflammation resolves. Monocytes are recruited to the inflammatory lesion where they differentiate into MØ. We term these monocyte-derived MØ ‘inflammation-associated’ to distinguish them from Resident MØ which are present throughout the inflammatory response and can renew during the resolution of inflammation by proliferation. Comparative analysis of the Mo and MØ populations (both ‘inflammation-associated’ and Resident MØ) identifies select genes expressed in subsets of ‘inflammation-associated’ and Resident MØ that play important roles in the resolution of inflammation and/or for immunity, including molecules involved in antigen presentation, cell cycle and others associated with ‘immaturity’ and MØ activation.
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| Overall design |
We purified monocyte and macrophage populations from the peritoneal cavity of C57BL/6 mice 4, 18, 72 and 168 hours after the induction of inflammation with intraperitoneal administration of zymosan (2x10^7 particles). We also purified tissue resident macrophages and Ly-6B+ bone marrow monocytes from naive mice as reference populations.
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| Contributor(s) |
Taylor PR, Rosas M, Giles PJ |
| Citation(s) |
24762537 |
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| Submission date |
Apr 14, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Peter Giles |
| E-mail(s) |
GilesPJ@Cardiff.ac.uk
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| Organization name |
Cardiff University
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| Street address |
Heath Park
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| City |
CARDIFF |
| ZIP/Postal code |
CF14 4XN |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (21)
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| GSM709379 |
F4/80(med-high), Ly-6B+ inflammation-associated macrophages_168 hours_biological rep 1 |
| GSM709380 |
F4/80(med-high), Ly-6B+ inflammation-associated macrophages_168 hours_biological rep 2 |
| GSM709381 |
F4/80(med-high), Ly-6B+ inflammation-associated macrophages_168 hours_biological rep 3 |
| GSM709382 |
F4/80(high), Ly-6B- inflammation-associated macrophages_168 hours_biological rep 1 |
| GSM709383 |
F4/80(high), Ly-6B- inflammation-associated macrophages_168 hours_biological rep 2 |
| GSM709384 |
F4/80(high), Ly-6B- inflammation-associated macrophages_168 hours_biological rep 3 |
| GSM709385 |
Ly-6B+, Ly-6G- inflammation-associated monocyte_4 hours_biological rep 1 |
| GSM709386 |
Ly-6B+, Ly-6G- inflammation-associated monocyte_4 hours_biological rep 2 |
| GSM709387 |
Ly-6B+, Ly-6G- inflammation-associated monocyte_4 hours_biological rep 3 |
| GSM709388 |
F4/80+, CD11b+ inflammation-associated macrophages_72 hours_biological rep 1 |
| GSM709389 |
F4/80+, CD11b+ inflammation-associated macrophages_72 hours_biological rep 2 |
| GSM709390 |
F4/80+, CD11b+ inflammation-associated macrophages_72 hours_biological rep 3 |
| GSM709391 |
Ly-6B+/low, Ly-6G- inflammation-associated monocyte/macrophage_18 hours_biological rep 1 |
| GSM709392 |
Ly-6B+/low, Ly-6G- inflammation-associated monocyte/macrophage_18 hours_biological rep 2 |
| GSM709393 |
Ly-6B+/low, Ly-6G- inflammation-associated monocyte/macrophage_18 hours_biological rep 3 |
| GSM709394 |
F4/80(high), CD11b(high) peritoneal resident macrophages_naive_biological rep 1 |
| GSM709395 |
F4/80(high), CD11b(high) peritoneal resident macrophages_naive_biological rep 2 |
| GSM709396 |
F4/80(high), CD11b(high) peritoneal resident macrophages_naive_biological rep 3 |
| GSM709397 |
Ly-6B+Ly-6G- bone marrow monocyte_biological rep 1 |
| GSM709398 |
Ly-6B+Ly-6G- bone marrow monocyte_biological rep 2 |
| GSM709399 |
Ly-6B+Ly-6G- bone marrow monocyte_biological rep 3 |
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| Relations |
| BioProject |
PRJNA138723 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28621_RAW.tar |
89.4 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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