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| Status |
Public on Dec 20, 2011 |
| Title |
Gene expression of Pseudomonas aeruginosa (Pae) delta PA1006/nbvF mutant in the absence and presence of nitrate |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
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| Summary |
The Pae PA1006/nbvF gene was found to be essential for nitrate utilization, biofilm maturation, and virulence. Microarrays were employed to assess global gene expression changes in response to NO3 compared to wild-type.
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| Overall design |
Cells were grown aerobically in the absence or presence of nitrate and the effect on global gene expression was determined; two to three biological replicates for each condition were analyzed.
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| Contributor(s) |
Filiatrault MJ, Tombline G, Wagner VE, VanAlst N, Rumbaugh K, Sokol P, Iglewski BH |
| Citation(s) |
23409004 |
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| Submission date |
Apr 19, 2011 |
| Last update date |
Feb 12, 2020 |
| Contact name |
Barbara H. Iglewski |
| E-mail(s) |
Barbara_Iglewski@URMC.Rochester.edu
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| Phone |
585-275-3402
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| Organization name |
University of Rochester
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| Department |
Microbiology and Immunology
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| Lab |
Iglewski Lab
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| Street address |
601 Elmwood Ave
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| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
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| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
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| Samples (10)
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| GSM711446 |
PAO1 delta PA1006 strain grown in NY media, replicate 1 |
| GSM711447 |
PAO1 delta PA1006 strain grown in NY media, replicate 2 |
| GSM711448 |
PAO1 wild-type strain grown in NY media, replicate 1 |
| GSM711449 |
PAO1 wild-type strain grown in NY media, replicate 2 |
| GSM711450 |
PAO1 delta PA1006 strain grown in NY+NO3 media, replicate 1 |
| GSM711451 |
PAO1 delta PA1006 strain grown in NY+NO3 media, replicate 2 |
| GSM711452 |
PAO1 delta PA1006 strain grown in NY+NO3 media, replicate 3 |
| GSM711453 |
PAO1 wild-type strain grown in NY+NO3 media, replicate 1 |
| GSM711454 |
PAO1 wild-type strain grown in NY+NO3 media, replicate 2 |
| GSM711455 |
PAO1 wild-type strain grown in NY+NO3 media, replicate 3 |
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| Relations |
| BioProject |
PRJNA139015 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28719_RAW.tar |
7.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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