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| Status |
Public on Dec 21, 2012 |
| Title |
Modulation of gene expression by complement protein C1q in amyloid-beta injured neurons |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Complement protein C1q is induced after injury in the brain and during Alzheimer's disease and has been shown to protect against amyloid-beta induced neuronal death. In this study, we used microarray approach to identify the pathways modulated by C1q that are associated with neuroprotection.
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| Overall design |
Immature rat cortical primary neurons are treated with fibrillar amyloid-beta peptides and/or C1q for 3h before RNA extraction and hybridization on rat Affymetrix microarrays.
Supplementary file: Processed/normalized, probe-level signal intensities from neurons treated with amyloid-beta or C1q. Median signal intensity used as global normalization method, done with JMP genomics (v5.0) software.
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| |
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| Contributor(s) |
Benoit ME, Tenner AJ |
| Citation(s) |
23150673 |
| |
| Submission date |
Apr 27, 2011 |
| Last update date |
Nov 06, 2013 |
| Contact name |
Marie Emeline Benoit |
| E-mail(s) |
mbenoit@uci.edu
|
| Organization name |
UC Irvine
|
| Department |
Molecular Biology and Biochemistry
|
| Lab |
Tenner lab
|
| Street address |
2419 McGaugh Hall
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM716236 |
amyloid-beta treated neurons, replicate 1 |
| GSM716237 |
amyloid-beta treated neurons, replicate 2 |
| GSM716238 |
amyloid-beta treated neurons, replicate 3 |
| GSM716239 |
amyloid-beta + C1q treated neurons, replicate 1 |
| GSM716240 |
amyloid-beta + C1q treated neurons, replicate 2 |
| GSM716241 |
amyloid-beta + C1q treated neurons, replicate 3 |
|
| Relations |
| BioProject |
PRJNA139915 |
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