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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 17, 2011 |
| Title |
LPS hypersensitivity of gp130 mutant mice is independent of elevated hematopoietic TLR4 signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The inflammatory mediator IL6 induced by LPS, which signals via TLR4, has been shown to feedback and augment TLR4 signaling when over-produced in LPS hypersensitive gp130F/F mice. The identity of the LPS/TLR4 responsive inflammatory signaling pathways and gene networks which are modulated by IL6 are unknown. Therefore, to understand the molecular consequences of gp130 hyperactivity in non-haemopoietic tissue on LPS-induced systemic inflammation, global gene expression profiling of livers was performed.
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| Overall design |
The mouse 22K compugen oligo array from the Adelaide Microarray Facility was used to investigate the effect of LPS treatment on the livers of wildtype and gp130F/F mutant mice. Livers of both wildtype mice and gp130F/F mice at 6hr post LPS injection and also control livers wer collected. Each experimental group had 3 independant mice. In total 12 arrays were performed with a total of three biological replicates. A common reference design was used for this experiment. RNA extracted from eight pooled 17.5dpc C57BL/6 mouse embryos was used as the reference sample. 6 wildytpe mice and 6 gp130F/F mice were used in this study, with 3 mice of each genotype treated with LPS.
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| Contributor(s) |
Jenkins BJ, Gould JA, Grennhill CJ, Ernst M, Mansell A, Hertzog PJ |
| Citation(s) |
21670738 |
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| Submission date |
May 16, 2011 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Jodee Ann Gould |
| E-mail(s) |
jodee.gould@monash.edu
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| Organization name |
Monash Institute of Medical Research
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| Lab |
Centre for Innate Immunity & Infectious Diseases
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| Street address |
27-31 Wright Street
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| City |
Clayton |
| State/province |
Victoria |
| ZIP/Postal code |
3168 |
| Country |
Australia |
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| Platforms (1) |
| GPL8331 |
Adelaide Micoarray Facility Compugen Oligolibrary Mouse 22K V4.0 |
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| Samples (12)
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| GSM724385 |
wild type liver control, replicate 1 |
| GSM724386 |
wild type liver control, replicate 2 |
| GSM724387 |
wild type liver control, replicate 3 |
| GSM724388 |
wild type liver treated LPS, replicate 1 |
| GSM724389 |
wild type liver treated LPS, replicate 2 |
| GSM724390 |
wild type liver treated LPS, replicate 3 |
| GSM724391 |
gp130F/F mutant liver control, replicate 1 |
| GSM724392 |
gp130F/F mutant liver control, replicate 2 |
| GSM724393 |
gp130F/F mutant liver control, replicate 3 |
| GSM724394 |
gp130F/F mutant liver treated LPS , replicate 1 |
| GSM724395 |
gp130F/F mutant liver treated LPS , replicate 2 |
| GSM724396 |
gp130F/F mutant liver treated LPS , replicate 3 |
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| Relations |
| BioProject |
PRJNA138647 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29311_Lowess.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
| GSE29311_RAW.tar |
18.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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