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| Status |
Public on May 23, 2011 |
| Title |
Expression data from PDGF driven mouse tumors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Background
Tumor heterogeneity is a major obstacle for finding effective treatment of Glioblastoma (GBM). Based on global expression analysis, GBM can be classified into distinct subtypes: Proneural, Neural, Classical and Mesenchymal. The signatures of these different tumor subtypes may reflect the phenotypes of cells giving rise to them.
However, the experimental evidence connecting any specific subtype of GBM to particular cells of origin is lacking. In addition, it is unclear how different genetic alterations interact with cells of origin in determining tumor heterogeneity. This issue cannot be addressed by studying end-stage human tumors.
Methodology/Principal Findings
To address this issue, we used retroviruses to deliver transforming genetic lesions to glial progenitors in adult mouse brain. We compared the resulting tumors to human GBM. We found that different initiating genetic lesions gave rise to tumors with different growth rates.
However all mouse tumors closely resembled the human Proneural GBM.
Comparative analysis of these mouse tumors allowed us to identify a set of genes whose expression in humans with Proneural GBM correlates with survival.
Conclusions/Significance
This study offers insights into the relationship between adult glial progenitors and Proneural GBM, and allows us to identify molecular alterations that lead to more aggressive tumor growth. In addition, we present a new preclinical model that can be used to test treatments directed at a specific type of GBM in future studies.
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| Overall design |
Gene expression profiling was performed on 20 tumors (12 Ptenf/f and 8 Ptenf/f; p53f/f) and 3 normal brains from mice. End stage tumors were used for expression array analysis. The platform used was Affymetrix GeneChip Mouse Genome 430A 2.0 Array. The microarray labeling, hybridization and quality controls were performed by following Affymetrix protocol.
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| Contributor(s) |
Lei L, Canoll P |
| Citation(s) |
21625383, 24637229 |
| |
| Submission date |
May 23, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Liang Lei |
| Organization name |
Columbia University
|
| Street address |
1130 St. Nicholas Ave Rm 1001
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (23)
|
| GSM729236 |
Tumor in Ptenf/f mice (1pten 4) |
| GSM729237 |
Tumor in Ptenf/f mice (1pten 5) |
| GSM729238 |
Tumor in Ptenf/f mice (1pten 6) |
| GSM729239 |
Tumor in Ptenf/f; p53f/f mice (1ptenp53 7) |
| GSM729240 |
Tumor in Ptenf/f; p53f/f mice (1ptenp53 8) |
| GSM729241 |
Tumor in Ptenf/f; p53f/f mice (1ptenp53 9) |
| GSM729242 |
Tumor in Ptenf/f; p53f/f mice (1ptenp53 10) |
| GSM729243 |
Tumor in Ptenf/f; p53f/f mice (1ptenp53 11) |
| GSM729244 |
Tumor in Ptenf/f mice (2pten 5F) |
| GSM729245 |
Tumor in Ptenf/f mice (2pten F7) |
| GSM729246 |
Tumor in Ptenf/f mice (2pten F9) |
| GSM729247 |
Tumor in Ptenf/f mice (2pten JM3) |
| GSM729248 |
Tumor in Ptenf/f mice (2pten M11) |
| GSM729249 |
Tumor in Ptenf/f mice (2pten YFP1) |
| GSM729250 |
Tumor in Ptenf/f; p53f/f mice (2ptenp53 F1S) |
| GSM729251 |
Tumor in Ptenf/f; p53f/f mice (2ptenp53 M1) |
| GSM729252 |
Tumor in Ptenf/f; p53f/f mice (2ptenp53 R3F) |
| GSM729253 |
Normal brain (2NB1F) |
| GSM729254 |
Normal brain (2NB2F) |
| GSM729255 |
Normal brain (2NB3F) |
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| Relations |
| BioProject |
PRJNA141577 |
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