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| Status |
Public on Aug 22, 2011 |
| Title |
Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation (ChIP-seq) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
SAGA is a highly conserved transcriptional co-activator complex involved in multiple steps of transcription with activities that function both pre and post initiation. Loss of individual subunits results in developmental defects, suggesting a role in development. To better understand the roles of SAGA functions in developmental gene expression and it's relationship with RNA polymerase II, we examined its composition, binding profile, and the effects of subunit loss on gene expression in two distinct cell types in late stage Drosophila embryos: muscle and neurons.
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| Overall design |
Chromatin IP of FLAG-tagged SAGA subunit Ada2b, and RNA polymerase II (antibody 4H8), was performed in neuronal (elav+) or muscle (mef2+) cells isolated by FACS from late stage embryos, and compared to whole cell extracts (input). The control constists of an IP performed in neuronal cells from a non-tagged strain.
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| Contributor(s) |
Weake VM, Seidel CW, Peak A, Workman JL |
| Citation(s) |
21764853 |
| |
| Submission date |
May 24, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Chris W Seidel |
| E-mail(s) |
seidel@phageT4.org
|
| Phone |
816 926 9054
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| Organization name |
Stowers Institute
|
| Department |
Genomics
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| Lab |
Seidel
|
| Street address |
1000 E 50th St
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
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| Platforms (1) |
| GPL9061 |
Illumina Genome Analyzer II (Drosophila melanogaster) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE29528 |
Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation |
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| Relations |
| SRA |
SRP006899 |
| BioProject |
PRJNA153999 |
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