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Series GSE29703 Query DataSets for GSE29703
Status Public on Aug 01, 2012
Title Association of microRNAs with CD68 and NOS2 in IBD tissues.
Platform organisms Homo sapiens; Mus musculus
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary We analyzed the association of CD68 and NOS2 mRNA expression with microRNAs in IBD tissues. For this analysis, data was LOESS normalized in R. Data was then imported into Partek Genomics Suite and Batch effects caused by day to day variability (Dategroup of array charateristic) were corrected. We did not have mRNA expression data for CD68 and NOS2 for all of the samples, therefore those samples were not used for analysis.
We identified several microRNAs associated with CD68 and NOS2 expression.
 
Overall design Total mRNA was extracted from colon tissue that was flash frozen immediately after surgery. A total of 114 tissues were used for microarray analysis. Briefly, 5 ug of RNA were biotin labeled and hybridized to OSU-CCC microRNA microarrays version 2.0. We then analyzed associations with CD68 and NOS2 mRNA expression.
 
Contributor(s) Schetter AJ, Sohn JJ, Harris CC
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Submission date Jun 02, 2011
Last update date Aug 02, 2012
Contact name Aaron Schetter
E-mail(s) schettera@mail.nih.gov
Organization name National Cancer Institute
Department Laboratory of Human Carcinogenesis
Street address 37 Convent Drive, Building 37, Room 3050
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL13681 OSU-CCC MicroRNA Microarray Version 2
Samples (114)
GSM736770 16467-01F
GSM736771 16468-01N
GSM736772 16469-02F
Relations
BioProject PRJNA141191

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29703_RAW.tar 50.9 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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