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Status |
Public on Aug 01, 2012 |
Title |
Association of microRNAs with CD68 and NOS2 in IBD tissues. |
Platform organisms |
Homo sapiens; Mus musculus |
Sample organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by array
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Summary |
We analyzed the association of CD68 and NOS2 mRNA expression with microRNAs in IBD tissues. For this analysis, data was LOESS normalized in R. Data was then imported into Partek Genomics Suite and Batch effects caused by day to day variability (Dategroup of array charateristic) were corrected. We did not have mRNA expression data for CD68 and NOS2 for all of the samples, therefore those samples were not used for analysis. We identified several microRNAs associated with CD68 and NOS2 expression.
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Overall design |
Total mRNA was extracted from colon tissue that was flash frozen immediately after surgery. A total of 114 tissues were used for microarray analysis. Briefly, 5 ug of RNA were biotin labeled and hybridized to OSU-CCC microRNA microarrays version 2.0. We then analyzed associations with CD68 and NOS2 mRNA expression.
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Contributor(s) |
Schetter AJ, Sohn JJ, Harris CC |
Citation missing |
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Submission date |
Jun 02, 2011 |
Last update date |
Aug 02, 2012 |
Contact name |
Aaron Schetter |
E-mail(s) |
schettera@mail.nih.gov
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Organization name |
National Cancer Institute
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Department |
Laboratory of Human Carcinogenesis
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Street address |
37 Convent Drive, Building 37, Room 3050
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City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
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Platforms (1) |
GPL13681 |
OSU-CCC MicroRNA Microarray Version 2 |
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Samples (114)
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Relations |
BioProject |
PRJNA141191 |