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| Status |
Public on Jul 01, 2012 |
| Title |
Comparison of Ets2-null and control ES cell derived lineages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Ets2-null ES cells are defective in differentiating into cardiac myocytes, evidenced by the lack of spontaneous beating, cardiac transcription factors and structural proteins. With the Phalanx mouse Onearray v2, we compared the gene expression profiles of cells derived from Ets2-null and wildtype ES cells.
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| Overall design |
Standard embryoid body culture protocol was used to induce differentiation of murine ES cells. On the 10th day, replicates of either Ets2-null or control wildtype EB culture were collected in Trizol for total RNA extraction. Microarray was preformed by Phalanx.
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| |
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| Contributor(s) |
Liu Y, Harger J |
| Citation(s) |
22826236 |
| Submission date |
Jun 03, 2011 |
| Last update date |
Aug 02, 2012 |
| Contact name |
Robert Joel Schwartz |
| E-mail(s) |
rjschwartz@uh.edu
|
| Phone |
832-630-1094
|
| Organization name |
University of Houston
|
| Department |
Biology and Biochemistry
|
| Lab |
Schwartz
|
| Street address |
4800 Calhoun
|
| City |
Houston Road |
| State/province |
Texas |
| ZIP/Postal code |
77204 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13684 |
Phalanx Biotech mouse OneArray Microarray v2 |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA141081 |
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