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| Status |
Public on Jan 01, 2016 |
| Title |
3 quorum sensing signals add in microarray comparison in Yersinia pestis at 30°C |
| Organism |
Yersinia pestis |
| Experiment type |
Expression profiling by array
|
| Summary |
The quorum-sensing system has been linked to diverse phenotypes and regulatory changes in pathogenic bacteria. In strain CO92, the AI-2 signal is produced in a luxS-dependent manner, reaching maximal levels of 2.5 μM in late logarithmic growth, and both wild type and pigmentation mutant strains made equivalent levels of AI-2. Yersinia pestis CO92 possesses a chromosomal lsr locus encoding factors involved in the binding and import of AI-2, and confirming this assignment, an lsr deletion increased extracellular pools of AI-2. To assess the functional role of 3 quorum sensing singlas in Y. pestis, microarray study was conducted comparing the 3 quorum sensing signals added in to control at 30°C to mimic the flea gut. Here, the ΔPgm (pigmentation-negative) R88 strain was used. The three signals are one AI-2 and two AHLs (N-(3-Oxooctanoyl)-L-homoserine lactone and N-Hexanoyl-DL-homoserine lactone).The control consisted of cells grown and treated under the same conditions without added signals.
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| Overall design |
Six independent RNA samples from 3 signals add in cultures were paired with six independent RNA samples from control cultures for hybridization to six two-color microarrays. A dye-swap design was used to remove the Cy5 and Cy3 dye bias.
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| Contributor(s) |
Minion C, Yu J |
| Citation(s) |
23959719 |
| |
| Submission date |
Jun 20, 2011 |
| Last update date |
Apr 01, 2016 |
| Contact name |
Chris Minion |
| E-mail(s) |
fcminion@iastate.edu
|
| Phone |
515-294-6347
|
| Fax |
515-294-8500
|
| Organization name |
Iowa State University
|
| Department |
VMPM
|
| Street address |
1130 Vet Med
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10017 |
WUSTL Yersinia pestis 15K array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE30342 |
AI-2 quorum sensing in *Yersinia pestis* |
|
| Relations |
| BioProject |
PRJNA155193 |
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