|
| Status |
Public on Nov 23, 2011 |
| Title |
σB-dependent yabJ-spoVG operon involved in regulation of extracellular nuclease, lipase and protease expression in Staphylococcus aureus |
| Platform organisms |
Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus USA300 |
| Sample organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by array
|
| Summary |
The alternative sigma factor σB of Staphylococcus aureus is involved in the coordination of the general stress response, expression of virulence determinants and modulation of antibiotic resistance levels. It controls a large regulon, either directly by recognizing conserved σB promoter sequences, or indirectly via σB-dependent elements. The σB-controlled yabJ-spoVG operon encodes two such putative downstream elements. We report here transcriptome analysis in S. aureus Newman showing that inactivation of the yabJ-spoVG operon had primarily a repressing effect on a small subregulon comprising mainly virulence factors, including a nuclease (nuc), a protease (splE) and a lipase (lip).. As a consequence extracellular nuclease, protease and lipase activities were reduced in a yabJspoVG mutant. Trans-complementation by SpoVG was sufficient to restore their reduced phenotypic expression and lowered transcription due to yabJ-spoVG deletion. It did not restore, however, the changes triggered by σB inactivation, indicating that both regulons do only partially overlap, despite the σB dependency of the yabJ-spoVG expression. Thus, σB is likely to control additional, SpoVG-independent factors affecting the expression of numerous hydrolytic enzymes. SpoVG, on the other hand, seems to fine-tune the σB-dependent regulation of a subset of virulence factors by antagonizing the σB effect.
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| Overall design |
Newman wild type strain compared to double mutant spoVG and yabJ or to sigB truncated mutant
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| Contributor(s) |
Schulthess B, Bloes DA, François P, Girard M, Schrenzel J, Bischoff M, Berger-Bächi B |
| Citation(s) |
21725011 |
| |
| Submission date |
Jun 20, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
FRANCOIS Patrice |
| E-mail(s) |
patrice.francois@genomic.ch
|
| Phone |
+41 (0)22 372 93 37
|
| Organization name |
Genomic Research Laboratory
|
| Department |
Service of Infectious Diseases
|
| Lab |
Genomic Research Lab
|
| Street address |
Rue Gabrielle-Perret-Gentil, 4
|
| City |
Geneva |
| State/province |
Ge 4 |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL7137 |
Agilent-017903 Staphylococcus aureus V5 Bis 15K (basic) |
|
| Samples (9)
|
| GSM745619 |
Wild type strain Newman replicate 1 |
| GSM745620 |
Wild type strain Newman replicate 2 |
| GSM745621 |
Wild type strain Newman replicate 3 |
| GSM745622 |
Newman mutant strain DyabJ-spoVG::erm(B) replicate 1 |
| GSM745623 |
Newman mutant strain DyabJ-spoVG::erm(B) replicate 2 |
| GSM745624 |
Newman mutant strain DyabJ-spoVG::erm(B) replicate 3 |
| GSM745625 |
Newman mutant strain sigB1(Am) replicate 1 |
| GSM745626 |
Newman mutant strain sigB1(Am) replicate 2 |
| GSM745627 |
Newman mutant strain sigB1(Am) replicate 3 |
|
| Relations |
| BioProject |
PRJNA144047 |
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