|
| Status |
Public on Jun 22, 2011 |
| Title |
Protein expression in primary murine dermal fibroblasts grown on fibronectin vs. plastic |
| Platform organism |
Homo sapiens |
| Sample organism |
Mus musculus |
| Experiment type |
Protein profiling by protein array
|
| Summary |
We were interested in identifying proteins that are regulated in response to the extracellular matrix molecule, fibronectin, in dermal fibroblasts. To determine proteins regulated by exposure to fibronectin, primary murine dermal fibroblasts were cultured on tissue culture plastic or on tissue culture plates coated with a thin layer of fibronectin (2 μg/cm2). Fibroblasts were seeded at 35,000 cells/10 cm2 and were harvested 48 hours later. Cell pellets from these fibroblasts were shipped to Kinexus Bioinformatics Corp. (Vancouver, B.C.) where they were lysed and analyzed using the Kinex TM Antibody Microarray (KAM 1.2), which contains 800 (500 pan-specific and 300 phosphorylation site-specific) antibodies. This enabled us to identify candidate proteins regulated by exposure to fibronectin.
|
| |
|
| Overall design |
Primary murine dermal fibroblasts were grown on fibronectin and tissue culture plastic for 48 hours. Cell pellets from these fibroblasts were shipped to Kinexus Bioinformatics Corp. (Vancouver, B.C.) where they were lysed and analyzed using the Kinexus Antibody Microarray (KAM 1.2), which contains 800 (500 pan-specific and 300 phosphorylation-site specific) antibodies.
|
| |
|
| Contributor(s) |
Alman BA, Bielefeld KA |
| Citation(s) |
21652705 |
| Submission date |
Jun 21, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Benjamin Alman |
| Organization name |
Hospital for Sick Children
|
| Department |
Developmental and Stem Cell Biology
|
| Lab |
Alman Lab
|
| Street address |
TMDT, 101 College Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL13757 |
Kinexus KAM-1.2FN 800 Ab microarray |
|
| Samples (1) |
| GSM746015 |
Fibroblasts - Fibronectin vs. Plastic |
|
| Relations |
| BioProject |
PRJNA143831 |
