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Series GSE30149 Query DataSets for GSE30149
Status Public on Jul 15, 2011
Title Global transcriptome analysis of two ameiotic1 alleles in maize anthers: defining steps in meiotic entry and progression through prophase
Organism Zea mays
Experiment type Expression profiling by array
Summary This study compared the transcriptomes of maize anthers at both 1mm and 1.5mm lengths from male sterile mutants with either the am1-489 or am1-pra alleles as well as from fertile siblings of the mutant plants.
Comparisons were done varying just the stage (1mm vs 1.5 mm), just the allele (am1-pra vs am1-489 at the same stage), and wild-type vs mutant (same stage and allele). Genes were categorized as On (expressed) or Off (not detected) and if both channels produced an intensity, the differential expression was determined. AM1 seems to be involved in establishing or stabilizing meiotic chromosomes and it had a profound impact on gene expression in the pollen mother cells during the maturation phase from 1.0 to 1.5 mm anther length. The two alleles shared some transcript changes but am1-489 showed a more dramatic loss (>3400) at 1.5mm than am1-pra. Most differentially expressed transcripts were allele-specific.
KEYWORDS: male sterility, maize, microarray, meiosis
 
Overall design Compared wild-type and male-sterile anthers from 2 ameiotic1 alleles at 2 stages of anther growth (1mm and 1.5mm)
 
Contributor(s) Nan G, Ronceret A, Wang RC, Fernandes JF, Cande WZ, Walbot V
Citation(s) 21867558
Submission date Jun 22, 2011
Last update date Aug 27, 2019
Contact name John F Fernandes
E-mail(s) jfernand@stanford.edu
Phone 650-533-9376
Organization name Stanford University
Department Bio Sci
Lab Walbot
Street address 385 Serra Mall
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL7444 Agilent-016047 Walbot Zea mays 4x44K array
Samples (64)
GSM746634 am1-489msE_489wtE-Rep1-Cy5-489msE
GSM746635 am1-489msE_489wtE-Rep2-Cy5-489wtE
GSM746636 am1-489msF_489wtF-Rep1-Cy5-489msF
Relations
BioProject PRJNA143857

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30149_RAW.tar 701.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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