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| Status |
Public on Jun 30, 2012 |
| Title |
Mechanisms of crystalline silica-induced pulmonary toxicity revealed by global gene expression profiling (rat lungs) |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
A proper understanding of the mechanisms underlying crystalline silica-induced pulmonary toxicity has implications in the management and potential prevention of the adverse health effects associated with silica exposure including silicosis, cancer and several auto-immune diseases. Human lung type II epithelial cells and rat lungs exposed to crystalline silica were employed as experimental models to determine global gene expression changes in order to understand the molecular mechanisms underlying silica-induced pulmonary toxicity. The differential gene expression profile induced by silica correlated with its toxicity in the A549 cells. The biological processes perturbed by silica exposure in the A549 cells and rat lungs, as identified by the bioinformatic analysis of the differentially expressed genes, demonstrated significant similarity. Functional categorization of the differentially expressed genes identified cancer, cellular movement, cellular growth and proliferation, cell death, inflammatory response, cell cycle, cellular development, and genetic disorder as top-ranking biological functions perturbed by silica exposure in the A549 cells and rat lungs. The involvement of oxidative stress and apoptosis in the silica-induced pulmonary toxicity was confirmed by ELISA and confocal microscopy analysis, respectively, of the silica-exposed A549 cells. Results of our study, in addition to confirming several previously identified molecular targets and mechanisms involved in silica toxicity, identified novel molecular targets and mechanisms potentially involved in silica-induced pulmonary toxicity. Further investigations, including those focused on the novel molecular targets and mechanisms identified in the current study, may result in a better management and, possibly, reduction and/or prevention of the potential adverse health effects associated with crystalline silica exposure.
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| Overall design |
20 samples were analyzed in this experiment. RNA from rat lung samples was isolated for gene expression studies. Rats were exposed to crystalline silica by inhalation (15 mg/m3, 6 hours/day, 5 days) or air. Lung gene expression profiling was performed using rat lung samples, 8 for silica exposed and 12 for air-exposed controls at 0 weeks post-exposure time period.
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| Contributor(s) |
Sellamuthu R, Umbright C, Li S, Kashon M, Pius J |
| Citation(s) |
22087542 |
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| Submission date |
Jun 23, 2011 |
| Last update date |
Mar 16, 2020 |
| Contact name |
Rajendran Sellamuthu |
| E-mail(s) |
rajsella@indiana.edu
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| Organization name |
National Institute for Occupational Safety and Health
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| Department |
HELD
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| Lab |
Molecular Carcinogenesis
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| Street address |
1095 Willowdale Rd
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| City |
Morgantown |
| State/province |
WV |
| ZIP/Postal code |
26505 |
| Country |
USA |
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| Platforms (1) |
| GPL6101 |
Illumina ratRef-12 v1.0 expression beadchip |
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| Samples (20)
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| GSM747203 |
Lung_15mg silica_0 week post-exposure_rep3 |
| GSM747204 |
Lung_0mg silica_0 week post-exposure_rep4 |
| GSM747205 |
Lung_15mg silica_0 week post-exposure_rep5 |
| GSM747206 |
Lung_0mg silica_0 week post-exposure_rep5 |
| GSM747207 |
Lung_0mg silica_0 week post-exposure_rep11 |
| GSM747208 |
Lung_0mg silica_0 week post-exposure_rep3 |
| GSM747209 |
Lung_15mg silica_0 week post-exposure_rep4 |
| GSM747210 |
Lung_0mg silica_0 week post-exposure_rep6 |
| GSM747211 |
Lung_15mg silica_0 week post-exposure_rep7 |
| GSM747212 |
Lung_0mg silica_0 week post-exposure_rep12 |
| GSM747213 |
Lung_15mg silica_0 week post-exposure_rep1 |
| GSM747214 |
Lung_0mg silica_0 week post-exposure_rep2 |
| GSM747215 |
Lung_15mg silica_0 week post-exposure_rep2 |
| GSM747216 |
Lung_0mg silica_0 week post-exposure_rep1 |
| GSM747217 |
Lung_15mg silica_0 week post-exposure_rep6 |
| GSM747218 |
Lung_0mg silica_0 week post-exposure_rep7 |
| GSM747219 |
Lung_0mg silica_0 week post-exposure_rep10 |
| GSM747220 |
Lung_0mg silica_0 week post-exposure_rep9 |
| GSM747221 |
Lung_0mg silica_0 week post-exposure_rep8 |
| GSM747222 |
Lung_15mg silica_0 week post-exposure_rep8 |
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| This SubSeries is part of SuperSeries: |
| GSE30216 |
Mechanisms of crystalline silica-induced pulmonary toxicity revealed by global gene expression profiling |
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| Relations |
| BioProject |
PRJNA155203 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30178_RAW.tar |
2.8 Mb |
(http)(custom) |
TAR |
| GSE30178_non-normalized.txt.gz |
5.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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