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Status |
Public on Jul 01, 2011 |
Title |
Analyses of the GlnR- and AmtR- dependent nitrogen regulatory network of Mycobacterium smegmatis. |
Platform organism |
Mycolicibacterium smegmatis MC2 155 |
Sample organism |
Mycolicibacterium smegmatis |
Experiment type |
Expression profiling by array
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Summary |
Total transcriptome analyses were carried out to investigate changes in transcript patterns in the Mycobacterium smegmatis wild type strain SMR5 due to nitrogen starvation and to expand the knowledge about the role of the two transcriptional regulators of nitrogen metabolism, namely GlnR and AmtR, in these processes. A first experiment revealed enhanced transcript levels of 284 genes and reduced transcripts of 231 genes in the wild type under nitrogen starvation compared to nitrogen surplus. When glnR deletion strain MH1 was compared to the wild type under nitrogen starvation, decreased transcript levels of 125 genes were detected, indicating that these are activated by GlnR due to nitrogen limitation. Comparing amtR deletion strain YL1 to the wild type under nitrogen starvation, enhanced transcript levels of 2 genes were found, indicating that they are repressed by AmtR under nitrogen surplus. A comparison of YL1 and wild type under surplus, as well as a comparison of YL1 under nitrogen surplus and starvation and of MH1 under nitrogen surplus and starvation were carried out as additional control experiments. It can be concluded that GlnR is the master regulator of nitrogen control in M. smegmatis and that AmtR fulfills only a small, subordinate role in the regulation of an operon.
This SuperSeries is composed of the SubSeries listed below.
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Overall design |
Refer to individual Series
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Citation(s) |
24266988 |
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Submission date |
Jun 27, 2011 |
Last update date |
Apr 25, 2023 |
Contact name |
Weber Simone |
E-mail(s) |
simoneweber.fr@gmail.com, Selina.Sitte@uk-erlangen.de
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Phone |
0049-9131-8535000
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Organization name |
Friedrich-Alexander University Erlangen-Nuremberg
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Department |
Medicine
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Lab |
Prof. R. Atreya
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Street address |
Ulmenweg 18
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City |
Erlangen |
State/province |
Bavaria |
ZIP/Postal code |
91052 |
Country |
Germany |
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Platforms (1) |
GPL13735 |
Agilent-026062 Msmeg_v1 (Probe Name version) |
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Samples (23)
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This SuperSeries is composed of the following SubSeries:
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GSE30033 |
Mycobacterium smegmatis - Comparison of glnR deletion strain MH1 under nitrogen surplus and starvation |
GSE30231 |
Mycobacterium smegmatis - Comparison of wild type SMR5 and amtR deletion strain YL1 under nitrogen starvation |
GSE30232 |
Mycobacterium smegmatis - Comparison of amtR deletion strain YL1 under nitrogen surplus and starvation |
GSE30233 |
Mycobacterium smegmatis - Comparison of wild type SMR5 under nitrogen surplus and starvation |
GSE30234 |
Mycobacterium smegmatis - Comparison of wild type SMR5 and glnR deletion strain MH1 under nitrogen starvation |
GSE30235 |
Mycobacterium smegmatis - Comparison of wild type SMR5 and amtR deletion strain YL1 under nitrogen surplus |
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Relations |
BioProject |
PRJNA143787 |