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Series GSE30236 Query DataSets for GSE30236
Status Public on Jul 01, 2011
Title Analyses of the GlnR- and AmtR- dependent nitrogen regulatory network of Mycobacterium smegmatis.
Platform organism Mycolicibacterium smegmatis MC2 155
Sample organism Mycolicibacterium smegmatis
Experiment type Expression profiling by array
Summary Total transcriptome analyses were carried out to investigate changes in transcript patterns in the Mycobacterium smegmatis wild type strain SMR5 due to nitrogen starvation and to expand the knowledge about the role of the two transcriptional regulators of nitrogen metabolism, namely GlnR and AmtR, in these processes. A first experiment revealed enhanced transcript levels of 284 genes and reduced transcripts of 231 genes in the wild type under nitrogen starvation compared to nitrogen surplus. When glnR deletion strain MH1 was compared to the wild type under nitrogen starvation, decreased transcript levels of 125 genes were detected, indicating that these are activated by GlnR due to nitrogen limitation. Comparing amtR deletion strain YL1 to the wild type under nitrogen starvation, enhanced transcript levels of 2 genes were found, indicating that they are repressed by AmtR under nitrogen surplus. A comparison of YL1 and wild type under surplus, as well as a comparison of YL1 under nitrogen surplus and starvation and of MH1 under nitrogen surplus and starvation were carried out as additional control experiments. It can be concluded that GlnR is the master regulator of nitrogen control in M. smegmatis and that AmtR fulfills only a small, subordinate role in the regulation of an operon.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series
 
Citation(s) 24266988
Submission date Jun 27, 2011
Last update date Apr 25, 2023
Contact name Weber Simone
E-mail(s) simoneweber.fr@gmail.com, Selina.Sitte@uk-erlangen.de
Phone 0049-9131-8535000
Organization name Friedrich-Alexander University Erlangen-Nuremberg
Department Medicine
Lab Prof. R. Atreya
Street address Ulmenweg 18
City Erlangen
State/province Bavaria
ZIP/Postal code 91052
Country Germany
 
Platforms (1)
GPL13735 Agilent-026062 Msmeg_v1 (Probe Name version)
Samples (23)
GSM743320 DglnR_without vs. with N_dyeswap_rep1
GSM743321 DglnR_without vs. with N_rep1
GSM743322 DglnR_without vs. with N_rep2
This SuperSeries is composed of the following SubSeries:
GSE30033 Mycobacterium smegmatis - Comparison of glnR deletion strain MH1 under nitrogen surplus and starvation
GSE30231 Mycobacterium smegmatis - Comparison of wild type SMR5 and amtR deletion strain YL1 under nitrogen starvation
GSE30232 Mycobacterium smegmatis - Comparison of amtR deletion strain YL1 under nitrogen surplus and starvation
Relations
BioProject PRJNA143787

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30236_RAW.tar 95.4 Mb (http)(custom) TAR (of TXT)

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