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| Status |
Public on Oct 01, 2011 |
| Title |
Mycobacterium tuberculosis H37Rv (WT) versus Mut1 and Comp1 |
| Organism |
Mycobacterium tuberculosis H37Rv |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptional profiling of H37Rv (WT), Mut1 and Comp1 bacteria under aerobic (Aer/0 day, i.e 0 D) and hypoxic conditions (Hyp/5 days standing culture, i.e 5 D). Mut1: H37Rv carrying devR gene disruption by in frame insertion of kanamycin resistance cassette and expressing DevRN-Kan fusion protein. Comp1: Mut1 complemented with low copy number plasmid carrying devR gene expressed from its native constitutive upstream promoter. (Reference: Majumdar et al., 2010, PLoS One 5:e9448). Goal is to compare transcriptional patterns of WT, Mut1 and Comp1 strains under aerobic (0 D) and hypoxic (5 D) conditions in vitro.
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| Overall design |
Two color and One-color experiments,Organism: Mycobacterium tuberculosis, Genotypic Technology designed Custom Mycobacterium tuberculosis H37Rv Whole Genome 8x15k GE Microarray (AMADID-020181)
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| Contributor(s) |
De Majumdar S, Sivaswami Tyagi J |
| Citation(s) |
22563409, 30755157 |
| |
| Submission date |
Jun 28, 2011 |
| Last update date |
Mar 06, 2019 |
| Contact name |
Genotypic technology |
| E-mail(s) |
sudha.rao@genotypic.co.in
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| Organization name |
Genotypic Technology
|
| Street address |
259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560094 |
| Country |
India |
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| Platforms (1) |
| GPL13729 |
Genotypic Technology designed Custom Mycobacterium tuberculosis H37Rv Whole Genome 8x15k GE (AMADID-020181) |
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| Samples (17)
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| GSM749780 |
Comp1_ Rep5 0D (Cy5) vs. Comp1_ Rep4 0D (Cy3) |
| GSM749781 |
Comp1_Rep1 0D (Cy5) vs. Mut1_Rep1 0D (Cy3) |
| GSM749782 |
Comp1_Rep1_5D (Cy5) vs. WT_Rep3 0D (Cy3) |
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| Relations |
| BioProject |
PRJNA143723 |
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