|
| Status |
Public on Sep 01, 2011 |
| Title |
Sperm methylation profiles reveal features of epigenetic inheritance and evolution in primates |
| Organisms |
Pan troglodytes; Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
During germ cell and preimplantation development, mammalian cells undergo nearly complete reprogramming of DNA methylation patterns. We profiled the methylomes of human and chimp sperm as a basis for comparison to methylation patterns of ES cells. While the majority of promoters escape methylation in both ES cells and sperm, the corresponding hypomethylated regions show substantial structural differences. Repeat elements are heavily methylated in both germ and somatic cells; however, retrotransposons from several subfamilies evade methylation more effectively during male germ cell development, while other subfamilies show the opposite trend. Comparing methylomes of human and chimp sperm revealed a subset of differentially methylated promoters and strikingly divergent methylation in retrotransposon subfamilies, with an evolutionary impact that is apparent in the underlying genomic sequence. Thus, the features that determine DNA methylation patterns differ between male germ cells and somatic cells, and elements of these features have diverged between humans and chimpanzees.
|
| |
|
| Overall design |
Genomic DNA form Human and Chimp (2 human individuals; 2 chimp individuals; individuals sequenced separately) mature sperm was isolated, bisulfite converted and sequenced using the Illumina G2 platform
|
| |
|
| Contributor(s) |
Molaro A, Hodges E, Fang F, Song Q, McCombie RW, Hannon G, Smith A |
| Citation(s) |
21925323 |
| |
| Submission date |
Jun 30, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew D Smith |
| E-mail(s) |
andrewds@usc.edu
|
| Organization name |
University of Southern California
|
| Department |
Molecular and Computational Biology
|
| Lab |
Smith Lab
|
| Street address |
1050 Childs Way
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
| GPL9378 |
Illumina Genome Analyzer II (Pan troglodytes) |
|
| Samples (4)
|
|
| Relations |
| SRA |
SRP007400 |
| BioProject |
PRJNA143655 |
Less...
More...