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| Status |
Public on Jul 05, 2012 |
| Title |
Indole-3-Acetaldehyde Inhibits Escherichia coli O157:H7 Biofilm Formation by Reducing Curli Formation |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
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| Summary |
Pathogenic biofilms have been associated with persistent infections due to their high resistance to antimicrobial agents. To identify non-toxic biofilm inhibitors for enterohemorrhagic Escherichia coli O157:H7, indole-3-acetaldehyde was used and reduced E. coli O157:H7 biofilm formation. Global transcriptome analyses revealed that indole-3-acetaldehyde most repressed two curli operons, csgBAC and csgDEFG, and induced tryptophanase (tnaAB) in E. coli O157:H7 biofilm cells. Electron microscopy showed that indole-3-acetaldehyde reduced curli production in E. coli O157:H7. Together, this study shows that Actinomycetales are an important resource of biofilm inhibitors as well as antibiotics.
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| Overall design |
For the microarray experiments, E. coli O157:H7 EDL933 was inoculated in 100 ml of LB in 250 ml flasks with overnight cultures that were diluted at 1:100. Cells were shaken with 10g of glass wool at 250 rpm and 37°C for 7 hrs. Cells were immediately chilled with dry ice and 95% ethanol (to prevent RNA degradation) for 30 sec before centrifugation in 50 ml centrifuge tubes at 13,000 g for 2 min; cell pellets were frozen immediately with dry ice and stored -80°C. RNA was isolated using Qiagen RNeasy mini Kit (Valencia, CA, USA). To eliminate DNA contamination, Qiagen RNase-free DNase I was used to digest DNA. RNA quality was assessed by Agilent 2100 bioanalyser using the RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, The Netherlands), and quantity was determined by ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., DE, USA).
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| Contributor(s) |
LEE J, LEE J, KIM Y, KIM C, CHO MH |
| Citation(s) |
22274708 |
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| Submission date |
Jul 06, 2011 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Jintae Lee |
| E-mail(s) |
jtlee@ynu.ac.kr
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| Phone |
82-53-810-2533
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| Organization name |
Yeungnam University
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| Department |
Chemical engineering
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| Lab |
Biotechnology
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| Street address |
214-1 Daedong
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| City |
Gyeongsan-Si |
| State/province |
Gyeongsangbuk-Do |
| ZIP/Postal code |
712-749 |
| Country |
South Korea |
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| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
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| Samples (2) |
| GSM754675 |
E. coli O157:H7 EDL933 in LB at 37oC for 7 hrs incubation with DMSO |
| GSM754676 |
E. coli O157:H7 EDL933 in LB at 37oC for 7 hrs incubation with indole-3-acetaldehyde |
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| Relations |
| BioProject |
PRJNA143391 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30424_2.EI3ld_vs._ECDG.xlsx.gz |
5.0 Mb |
(ftp)(http) |
XLSX |
| GSE30424_RAW.tar |
1.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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