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Series GSE30586 Query DataSets for GSE30586
Status Public on Sep 23, 2011
Title An Extended dsRBD with a Novel Zinc-Binding Motif Mediates Nuclear Retention of Fission Yeast Dicer
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by genome tiling array
Summary Dicer proteins function in RNA interference (RNAi) pathways by generating small RNAs (sRNAs). Here we report the solution structure of the C-terminal domain of Schizosaccharomyces pombe Dicer (Dcr1). The structure reveals an unusual double-stranded RNA binding domain (dsRBD) fold embedding a novel zinc-binding motif that is conserved among dicers in yeast. Although the C-terminal domain of Dcr1 still binds nucleic acids, this property is dispensable for proper functioning of Dcr1. In contrast, disruption of zinc coordination renders Dcr1 mainly cytoplasmic and leads to remarkable changes in gene expression and loss of heterochromatin assembly. In summary, our results reveal novel insights into the mechanism of nuclear retention of Dcr1 and raise the possibility that this new class of dsRBDs might generally function in nucleo-cytoplasmic trafficking and not substrate binding. The C-terminal domain of Dcr1 constitutes a novel regulatory module that might represent a potential target for therapeutic intervention with fungal diseases.
Overall design 12 chips representing 4 different strains. Every condition is represented by three biological replicates
Contributor(s) Barraud P, Emmerth S, Shimada Y, Hotz H, Allain FH, Bühler M
Citation(s) 21847092
Submission date Jul 12, 2011
Last update date Mar 23, 2012
Contact name Stephan Emmerth
Organization name FMI
Lab Bühler
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL10187 Affymetrix S. pombe Tiling 1.0FR Array [custom CDF: Sp20b_M_v04]
Samples (12)
GSM758776 wild-type tiling array 1
GSM758777 wild-type tiling array 2
GSM758778 wild-type tiling array 3
BioProject PRJNA144571

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30586_RAW.tar 144.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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