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Status |
Public on Oct 04, 2005 |
Title |
Identification of genes dependent on the Ecdysone receptor (EcR) at the onset of metamorphosis in Drosophila |
Organism |
Drosophila melanogaster |
Experiment type |
Expression profiling by array
|
Summary |
This study identifies those genes that are dependent on EcR for their proper regulation at the onset of metamorphosis in Drosophila melanogaster. Keywords: Nuclear receptor gene regulation
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Overall design |
hs-EcRi-11 third instar larvae were heat-treated twice at 37°C, each time for 1 hour, at 24 hours and 18 hours prior to pupariation, as described [29]. RNA was harvested for microarray analysis at -4, 0, and 4 hours relative to pupariation from three independent collections of animals for each time point. Data obtained from these arrays was compared to array data from control animals at the same stages of development to identify EcR-dependent genes.
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Contributor(s) |
Beckstead RB, Lam G, Thummel CS |
Citation(s) |
16356271 |
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Submission date |
Aug 05, 2005 |
Last update date |
Jul 06, 2016 |
Contact name |
Robert B Beckstead |
E-mail(s) |
robertb@uga.edu
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Phone |
706-542-0387
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Fax |
706-542-1827
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Organization name |
The University of Georgia
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Department |
Poultry Science
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Lab |
Robert B. Beckstead
|
Street address |
119 Poultry Science Building
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City |
Athens |
State/province |
GA |
ZIP/Postal code |
30602-2772 |
Country |
USA |
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Platforms (1) |
GPL72 |
[DrosGenome1] Affymetrix Drosophila Genome Array |
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Samples (18)
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GSM67390 |
third instar larvae -4 hours relative to pupariation EcRi control replicate 1 |
GSM67391 |
third instar larvae -4 hours relative to pupariation EcRi control replicate 2 |
GSM67392 |
third instar larvae -4 hours relative to pupariation EcRi control replicate 3 |
GSM67393 |
0 hour prepupae EcRi control replicate 1 |
GSM67394 |
0 hour prepupae EcRi control replicate 2 |
GSM67395 |
0 hour prepupae EcRi control replicate 3 |
GSM67396 |
4 hour prepupae EcRi control replicate 1 |
GSM67397 |
4 hour prepupae EcRi control replicate 2 |
GSM67398 |
4 hour prepupae EcRi control replicate 3 |
GSM67399 |
third instar larvae -4 hours relative to pupariation EcRi mutant replicate 1 |
GSM67400 |
third instar larvae -4 hours relative to pupariation EcRi mutant replicate 2 |
GSM67401 |
third instar larvae -4 hours relative to pupariation EcRi mutant replicate 3 |
GSM67402 |
0 hour prepupae EcR mutant replicate 1 |
GSM67403 |
0 hour prepupae EcR mutant replicate 2 |
GSM67404 |
0 hour prepupae EcR mutant replicate 3 |
GSM67405 |
4 hour prepupae EcR mutant replicate 1 |
GSM67406 |
4 hour prepupae EcR mutant replicate 2 |
GSM67407 |
4 hour prepupae EcR mutant replicate 3 |
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Relations |
BioProject |
PRJNA93165 |