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| Status |
Public on Aug 02, 2011 |
| Title |
Pyronaridine and chloroquine responses in the K1 strain |
| Organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by array
|
| Summary |
Pyronaridine (PN) and chloroquine (CQ) are structurally related anti-malarial drugs with primarily the same mode of action. However, PN is effective against several multidrug-resistant lines of Plasmodium falciparum, including CQ-resistant lines, suggestive of important operational differences between the two drugs.
Synchronized trophozoite-stage cultures of P. falciparum strain K1 (CQ resistant) were exposed to 50% inhibitory concentrations (IC50) of PN and CQ, and parasites were harvested from culture after 4 and 24 hours exposure. Global transcriptional changes effected by drug treatment were investigated using DNA microarrays.
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| Overall design |
Plasmodium falciparum in vitro cultures were synchronized to trophozoite stage (22-24h post infection) and exposed to either CQ or PN at IC50 concentrations. 18 sample pairs (drug treated/untreated) were analyzed; 9 for CQ and 9 for PN. All drug-treated samples were labelled with Cy5 and untreated controls were labelled with Cy3.
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| Contributor(s) |
Kritsiriwuthinan K, Chaotheing S, Shaw PJ, Wongsombat C, Chavalitshewinkoon-Petmitr P, Kamchonwongpaisan S |
| Citation(s) |
21849091 |
| |
| Submission date |
Jul 22, 2011 |
| Last update date |
Sep 18, 2014 |
| Contact name |
Philip James Shaw |
| E-mail(s) |
philip@biotec.or.th
|
| Organization name |
National Science and Technology Development Agency
|
| Department |
National Center for Genetic Engineering and Biotechnology
|
| Lab |
Protein and Molecular Biology
|
| Street address |
113 Phahonyothin Road
|
| City |
Pathumthani |
| State/province |
Pathumthani |
| ZIP/Postal code |
12120 |
| Country |
Thailand |
| |
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| Platforms (1) |
| GPL13818 |
BIOTEC P. falciparum 8448 features |
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| Samples (18)
|
| GSM765828 |
CQ 24h, replicate 1 |
| GSM765829 |
CQ 24h, replicate 2 |
| GSM765830 |
CQ 24h, replicate 3 |
| GSM765831 |
CQ 24h, replicate 4 |
| GSM765832 |
CQ 24h, replicate 5 |
| GSM765833 |
CQ 24h, replicate 6 |
| GSM765834 |
PN 4h, replicate 1 |
| GSM765835 |
PN 4h, replicate 2 |
| GSM765836 |
PN 4h, replicate 3 |
| GSM765837 |
PN 4h, replicate 4 |
| GSM765838 |
PN 24h, replicate 1 |
| GSM765839 |
PN 24h, replicate 2 |
| GSM765840 |
PN 24h, replicate 3 |
| GSM765841 |
PN 24h, replicate 4 |
| GSM765842 |
PN 24h, replicate 5 |
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| This SubSeries is part of SuperSeries: |
| GSE31109 |
Global gene expression profiling of Plasmodium falciparum in response to the anti-malarial drug pyronaridine |
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| Relations |
| BioProject |
PRJNA154593 |
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