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Status |
Public on Jun 18, 2012 |
Title |
Nucleosome occupancy in yeast BY4741and a strain lacking MSN2 and MSN4 responding to 20 min treatment with 0.4mM H2O2 |
Platform organism |
Saccharomyces cerevisiae |
Sample organism |
Saccharomyces cerevisiae S288C |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
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Summary |
Numerous factors have been implicated in regulating gene expression changes, including changes to nucleosome occupancy. Here we followed dynamic changes to nucleosome occupancy, gene expression and DNA binding of the transcription factor Msn2p genome-wide in yeast cells responding to hydrogen peroxide and reveal new relationships between regulators of stress-dependent gene expression in yeast.
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Overall design |
Nucleosome occupancy was measured in the S288c derivative BY4741 and a strain lacking MSN2 and MSN4. Nucleosomes were isolated from unstressed yeast and yeast treated for 20 min with 0.4mM H2O2. Nucleosomal samples were compared in a 2-color, competitive hybridization to sheared genomic DNA.
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Contributor(s) |
Huebert DJ, Gasch AP |
Citation(s) |
22354995 |
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Submission date |
Jul 25, 2011 |
Last update date |
Jul 07, 2012 |
Contact name |
Dana J Huebert |
Organization name |
University of Wisconsin-Madison
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Department |
Genetics
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Lab |
Audrey Gasch
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Street address |
425G Henry Mall
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City |
Madison |
State/province |
WI |
ZIP/Postal code |
53706 |
Country |
USA |
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Platforms (1) |
GPL9529 |
UW-Madison Saccharomyces cerevisiae 380K tiling array 51mer oligos 2006-10-12 |
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Samples (6)
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GSM766185 |
MNase_yeast_0.4mM_H2O2_wildtype_0min |
GSM766186 |
MNase_yeast_0.4mM_H2O2_wildtype_20min |
GSM766187 |
MNase_yeast_0.4mM_H2O2_wildtype_0min-rep2 |
GSM766188 |
MNase_yeast_0.4mM_H2O2_wildtype_20min-rep2 |
GSM766189 |
MNase_yeast_0.4mM_H2O2_msn24_0min |
GSM766190 |
MNase_yeast_0.4mM_H2O2_msn24_20min |
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This SubSeries is part of SuperSeries: |
GSE30901 |
Dynamic changes to nucleosome occupancy and genomic expression in yeast responding to oxidative stress |
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Relations |
BioProject |
PRJNA154577 |