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| Status |
Public on Oct 31, 2011 |
| Title |
Widespread Generation of Alternative UTRs Contributes to Sex-specific RNA Binding by UNR |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
Upstream of N-ras (UNR) is a conserved RNA-binding protein that regulates mRNA translation and stability by binding to sites generally located in untranslated regions (UTRs). In Drosophila, sex-specific binding of UNR to various RNAs plays key roles in the control of X chromosome dosage compensation in both sexes. In order to investigate broader sex-specific functions of UNR, we have identified its RNA targets in adult male and female flies by high-throughput RNA binding and transcriptome analysis. Here we show that UNR binds to a large set of protein-coding transcripts and to a smaller set of non-coding RNAs in a sex-specific fashion. The analyses also reveal a strong correlation between sex-specific binding of UNR and sex-specific differential expression of UTRs in target genes. Validation experiments indicate that UNR indeed recognizes sex-specifically processed transcripts. These results suggest that UNR exploits the transcript diversity generated by alternative processing and alternative promoter usage to bind and regulate target genes in a sex-specific manner.
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| Overall design |
Of the total 6 chips, three are independent immunoprecipitations, while the other three microarrays are their dye-swaps (same IPs).
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| Contributor(s) |
Mihailovich M, Würth L, Zambelli F, Abaza I, Militti C, Mancuso FM, Roma G, Pavesi G, Gebauer F |
| Citation(s) |
22101243 |
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| Submission date |
Jul 26, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Francesco Mattia Mancuso |
| E-mail(s) |
francesco.mancuso@crg.eu
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| Organization name |
CRG (Centre for Genomic Regulation)
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| Street address |
C/ Dr. Aiguader, 88
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| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL3797 |
01_Drosophila_16K_Universitat_Barcelona |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA146223 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30963_RAW.tar |
7.8 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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